Marten Smidt chairs the Molecular Neuroscience research team

Within that team the Smidtlab focuses on fundamental processes of brain development and has especially expertise on the molecular programming on midbrain dopaminergic neurons and their role in Parkinson's Disease. 

The expertise has lead to the development of a master program on Molecular Neurosciences here at the UVA, being part of the Neurobiology cluster of Biomedical sciences master programmes

Current research lines within the molecular programming of dopaminergic neurons:

1. Investigate the role of epigentic mechanisms on molecular specification of mdDA neurons
2. Understand the role of critical transcription factors in cortical development
3. Investigate mechanisms of dopamine production modulation.
4. Investigate mechanisms of specific cell death as found in SNc neurons.

5. Investigate the molecular details of TCF4 function in the brain in relation to Pitt Hopkins Syndrome

Research background

My main interest is molecular programming during development of the central nervous system. Since at that time almost nothing was known on the subject, I started with JPH Burbach a cloning effort to identify transcription factors that play a role during CNS development. From this work three major breakthroughs were established:
1. The cloning, identification and characterization of the homeobox gene Pitx3 [Smidt MP pnas 1997]. This gene was found to beexclusively expressed in the substantia nigra (SNc) and ventral tegmental area (VTA) forming the midbrain dopamine system, essential for mood and movement regulation in mammals. The real impact of the work was that this factor is exclusively expressed in these neurons and this formed the first step in the understanding of molecular programming of midbrain dopamine neurons.
2. The identification of the role of the orphan nuclear hormone receptor Nurr1  [Saucedo-Cardenas pnas 1998]. It was shown by us and others that Nurr1 is essential for the development of fully differentiated midbrain dopamine neurons. This was the second factor identified to be involved in midbrain dopamine neurodevelopment.
3. The identification of Lmx1b (publication [Smidt MP nat-neurosc.-2000]. We showed that the early specification of the midbrain by Lmx1b is essential for the formation of midbrain dopaminergic neurons. Moreover, we showed that TH expression was possible in early DA neurons without the expression of Pitx3, indicating that Pitx3 might be involved in molecular processes other than transmitter phenotype. 
After this cloning and descriptive phase I was interested to go into depth into the role of Pitx3 (as an NWO fellow) in the neurodevelopment of midbrain dopamine neurons and to broaden my view on molecular signaling events in neuronal cells. This was accomplished by the start of analysis of the Pitx3 knock out and by the start of a new research line on forkhead proteins (FoxO and FoxK family). The work lead to three major findings:
1. The data from the Pitx3 knock-out analysis (aphakia mouse) formed anew chapter in the understanding of the role of Pitx3 in the development of midbrain dopamine neurons (publication [enu:Smidt-MP development-2004]). We showed that Pitx3 is essential for the formation of the SNc and the ventral part of the VTA. Moreover it was clear from the analysis that the defect was apparent at the early stage of terminal differentiation, Most importantly, the defect was not present in all midbrain dopamine neurons but mainly in the ventral medial SNc and ventral VTA.
2. The cloning and functional characterization of FoxO6, a novel member of the FoxO family (FoxO1, FoxO3 and FoxO4). We showed that FoxO6 had unique properties in terms of nucleo-cytoplasmic shuttling, as a result of PKB signaling, compared to its family members  [Jacobs FM jbc2003]. Later we showed the mechanism of the altered shuttling behavior and showed that phosphorylation by PKB does inactivate FoxO6 by inhibiting DNA interaction without the extra level of inhibition of nuclear removal (publication [enu:van-der-Heide BJ 2005]). We have gained quickly a good position by this work. A review from our team received much enthusiasm as highlighted by 82 citations so-far and the 25th place of most downloaded papers in the publication year [Van-Der-Heide BJ review 2004].
3. The identification a novel factor of the FoxK family, namely (mouse) Foxk2. This factor was, based on sequence homology, the designated orthologue of yeast Fkh2 which is essential in the progression of the cell-cycle (G2/M phase progression). Our analysis showed that the functional similarity is not apparent, Foxk2 has a function in cell-survival but does not stimulate the G2/M phase transition.
The fact that a subset of the complete group of dopamine neurons was affected in the Pitx3 knock-out (Pitx3 itself is expressed in all midbrain dopamine cells) initiated the novel idea that the molecular coding of the SNc and VTA is not the same. A real step forward was madeby the identification of subset specific markers and coding differences in the ventricular zone. This concept of subset specification was a crucial step in the way the field regarded the molecular pathways leading to the development of dopamine neurons of the SNc and VTA [Smits SM prog neurobiol-2006]. In addition, it became clear that the neurons populating the SNc and VTA are not generated exclusively in the midbrain but also in the diencephalon. Therefore, the name of this neuronal group was changed to mesodiencephalic dopaminergic (mdDA) neurons and has been used as such from that point on.
This was a crucial moment in my career, i established my own group and my work clearly surpassed the initial identification and function-description of transcription factors involved in the development of mdDA neurons. My interest went into two additionally research directions:
1. The live visualization of dopamine neurotransmission through multi-pinhole SPECT. Prof dr. Freek Beekman designed a spectacular micro-SPECT system to follow tracers in the mouse brain with a resolution of 0.3 mm. Together we started to image live dopamine transmitter events in a living mouse. This work has lead to two high impact publications [Beekman FJ nucl medicin 2005] and [Vastenhouw B mol psych 2007]. 
2. Understand how subset specification is established, which subsets exist and what the relationship is between subset and specific connectivity. A crucial finding in the understanding of subsets specific molecular coding was established through the identification of a subset specific transcriptional target of Pitx3. This gene, (retinal) aldehyde dehydrogenase 2 (Ahd2, Raldh1) is expressed, in the adult brain, in the ventral cells of the SNc and VTA, the neurons that are lost in the Pitx3 mutant  [Jacobs FM development 2007]. A second novel finding was that retinoic acid (RA) has a role in terminal differentiation of this specific set of neurons in addition to its role in the ventricularzone. Ahd2 is present in ventricular zone cells, its transcription is terminated when cells leave the ventricular zone and under the control of Pitx3, Ahd2 is transcribed again in a (lateral) subset of mdDA neurons and is essential for the synthesis of RA out of retinal. We were able to show the dependence of mdDA differentiation for RA signalling by rescuing the Pitx3 knock-out phenotype through the exogenous application of RA. 
Although I am still working on- and interested in the FoxO6 knock out (we have generated chimeric animals) my main interest lies with the molecular programming of mdDA neuronal subsets. My lab is currently investigating 1) why Ahd2 activation is only happening in the described subset; 2) we are mapping transcriptional targets of Engrailed, Lmx1a/b, and Nurr1 to get a better idea about the molecular programming that is initiated within mdDA neurons; 3) the functional interaction between Pitx3/Nurr1 and En1 and 4) we are finalizing the identification of adult subset specific markers. At this moment my research group consist of 3 PhD students, 2 technicians and every year about 3 master students.
The work described above has been very fruit-full and has lead to a leading position of my research group mainly in the field of development and engineering of mdDA neurons, marked by many invitations to write reviews/books on the topic, the high amount of peer-reviewed publications and the invitations to speak at the international level about my work. I have initiated a European collaboration called mdDANeurodev (see www.mdDAneurodev.eu) which was funded in the KP7 scheme (2008) and in addition i was awarded a VICI grant from NWO-ALW (2008/2009) to continue the fundamental work on the development of subsets of dopamine neurons.

IN 2017 I founded with Dr. Lars vd Heide a biotech company: Macrobian Biotech BV. Mission : 

Fundamental research in the neuroscience field with new technologies in genetics and transcriptomics together with far reaching gene function research has led to the discovery of mechanistic details that is unprecedented. The founders of Macrobian-Biotech are at the heart of such endeavors and that has lead to the identification of specific molecular pathways that regulate dopamine production specifically in midbrain dopamine neurons. Dysfunction of these neurons are at the center of neuro-psychiatric and neurological disorders such as Schizophrenia and Parkinson‘s. It is the ambition of Macrobian-Biotech to be at the forefront of clinical trial ready compound development vivifying novel treatments of neurological and neuropsychiatric diseases. 

please see Macrobianbiotech.com for more details.

List of peer reviewed publications:

1. Neuronal Dot1l is a broad mitochondrial gene-repressor associated with human brain aging via H3K79 hypermethylation H.J Van Heesbeen, L Von Oerthel, P.M De Vries, M.R.J Wagemans, M.P. Smidt (2023) Int J Mol Sci.24(2):1387. doi: 10.3390/ijms24021387. IF 6.3
2. Molecular Organization and Patterning of the Medulla Oblongata in Health and Disease (2022) Dina Diek , Marten P Smidt and Simone Mesman. Int. J. Mol. Sci. 2022, 23(16), 9260; https://doi.org/10.3390/ijms23169260
3. Correction to: Survival of midbrain dopamine neurons depends on the Bcl2 factor Mcl1 Edward J. Robinson, Sebastian P. Aguiar, Willemieke M. Kouwenhoven, Dorinde S. Starmans, Lars von Oerthel, Marten P. Smidt, and Lars P. van der Heide. Cell Death Discov. 2022; 8: 102. doi: 10.1038/s41420-022-00871-3. IF 8.5
4. Mice hypomorphic for Pitx3 show robust entrainment of circadian behavioral and metabolic rhythms to scheduled feeding (2022), Lori L Scarpa, Brad Wanken, Marten Smidt, Ralph E Mistlberger, Andrew D Steele, Cell Rep . 2022 Jan 11;38(2):109865. doi: 10.1016/j.celrep.2021.109865. IF 8
5. The continued need for animals to advance brain research (2021)Judith R Homberg , Roger A H Adan , Natalia Alenina , Antonis Asiminas, Michael Bader, Tom Beckers, Denovan P Begg, Arjan Blokland , Marilise E Burger , Gertjan van Dijk , Ulrich L M Eisel , Ype Elgersma, Bernhard Englitz, Antonio Fernandez-Ruiz, Carlos P Fitzsimons, Anne-Marie van Dam, Peter Gass , Joanes Grandjean, Robbert Havekes, Marloes J A G Henckens, Christiane Herden, Roelof A Hut, Wendy Jarrett, Kate Jeffrey, Daniela Jezova, Andries Kalsbeek, Maarten Kamermans, Martien J Kas, Nael Nadif Kasri , Amanda J Kiliaan, Sharon M Kolk, Aniko Korosi, S Mechiel Korte, Tamas Kozicz, Steven A Kushner, Kirk Leech, Klaus-Peter Lesch, Heidi Lesscher, Paul J Lucassen, Anita Luthi, Liya Ma, Anne S Mallien, Peter Meerlo, Jorge F Mejias, Frank J Meye, Anna S Mitchell, Joram D Mul, Umberto Olcese, Azahara Oliva González, Jocelien D A Olivier, Massimo Pasqualetti, Cyriel M A Pennartz, Piotr Popik, Jos Prickaerts, Liset M de la Prida, Sidarta Ribeiro, Benno Roozendaal, Janine I Rossato, Ali-Akbar Salari, Regien G Schoemaker, August B Smit, Louk J M J Vanderschuren, Tomonori Takeuchi, Rixt van der Veen, Marten P Smidt, Vladyslav V Vyazovskiy, Maximilian Wiesmann, Corette J Wierenga, Bella Williams, Ingo Willuhn, Markus Wöhr, Monique Wolvekamp, Eddy A van der Zee andLisa Genzel. Neuron. 2021 Aug 4;109(15):2374-2379. doi: 10.1016/j.neuron.2021.07.015. PMID: 34352213. IF 17
6. Tcf4 Is Involved in Subset Specification of Mesodiencephalic Dopaminergic Neurons (2021) Simone Mesman , Iris Wever and Marten P. Smidt. Biomedicines 9(3), 317; https://doi.org/10.3390/biomedicines9030317. IF 5
7. Cue and reward evoked dopamine activity is necessary for maintaining learned Pavlovian associations. (2021) Ruud van Zessen, Jacques Flores, Timon Eekel, Siem van den Reijen, Bart Lodder, Azar Omrani, Marten Smidt, Geoffrey van der Plasse, Geert Ramakers, Garret Stuber, and Roger Adan. J Neurosci. Apr 19;JN-RM-2744-20. doi: 10.1523/JNEUROSCI.2744-20.2021. IF 6
8. ZNF91 deletion in human embryonic stem cells leads to ectopic activation of SVA retrotransposons and up-regulation of KRAB zinc finger gene clusters. (2021) Haring NL, van Bree EJ, Jordaan WS, Roels JRE, Sotomayor GC, Hey TM, White FTG, Galland MD, Smidt MP, Jacobs FMJ. Genome Res. 2021 Mar 15. doi: 10.1101/gr.265348.120. Online ahead of print. PMID: 33722937 IF 9
9. Advancing urban mental health research: from complexity to action (2021) J.M. van der Wal, C.D. van Borkulo, M. Deserno, J. J. F. Breedvelt, M. Lees, C. Lokman, Prof. D. Borsboom, Prof. D. Denys, R. van Holst, Prof. M. Smidt, Prof. K. Stronks, Prof. P.J. Lucassen, Prof. J.C.M. van Weert, Prof. P.M.A. Sloot, Prof. C.L.H. Bockting, & Prof. R.W. Wiers. Lancet Psychiatry https://doi.org/10.1016/S2215-0366(21)00047-X. IF 27
10. Tcf4 encodes cortical differentiation during development. (2020) Simone Mesman, Reinier Bakker, Marten Smidt. MCN Volume 106, July 2020, 103502, https://doi.org/10.1016/j.mcn.2020.103502. IF 3
11. Acquisition of the Midbrain Dopaminergic Neuronal Identity. (2020) Mesman S, Smidt MP. Int J Mol Sci. 2020 Jun 30;21(13):4638. doi: 10.3390/ijms21134638. PMID: 32629812. IF 6
12. MCL1 as a Therapeutic Target in Parkinson's Disease? (2019) Edward J.Robinson, SebastianAguiar, Marten P.Smidt and Lars P.van der Heide. Trends Mol. Med. 25(12):1056-1065. https://doi.org/10.1016/j.molmed.2019.08.009. IF 11
13. Characterization of transgenic mouse models targeting neuromodulatory systems reveals organizational principles of the dorsal raphe. (2019) Daniel Cardozo Pinto, Hongbin Yang, Iskra Pollak Dorocic, Johannes de Jong, Vivian Han, James Peck, Yichen Zhu, Christine Liu, Kevin Beier, Marten P. Smidt, and Stephan Lammel. Nat. Comm. 10:4633; https://doi.org/10.1038/s41467-019-12392. IF 15
14. Lmx1b Influences Correct Post-mitotic Coding of Mesodiencephalic Dopaminergic Neurons. (2019) Iris Wever, Pablo Largo-Barrientos, Elisa J. Hoekstra and Marten P. Smidt, Front. Mol. Neurosci., 14 March 2019 | https://doi.org/10.3389/fnmol.2019.00062. IF 6
15. EZH2 is essential for fate determination in the mammalian Isthmic area. (2019) Iris Wever, Cindy m. Wagemans and Marten P. Smidt. Front. Mol. Neurosci. | doi: 10.3389/fnmol.2019.00076 IF 6
16. Entanglement of Genetics and Epigenetics in Parkinson’s Disease (2019) H. J. van Heesbeen and Marten P. Smidt, Front. Neurosci. | doi: 10.3389/fnins.2019.00277. IF 4
17. EZH2 Influences mdDA Neuronal Differentiation, Maintenance and Survival.(2019) Wever I, von Oerthel L, Wagemans CMRJ, Smidt MP. Front Mol Neurosci. 2019 Jan 17;11:491. doi: 10.3389/fnmol.2018.00491. eCollection 2018. IF 6
18. Survival of midbrain dopamine neurons depends on the Bcl2 factor Mcl1. (2018) Robinson EJ, Aguiar SP, Kouwenhoven WM, Starmans DS, von Oerthel L, Smidt MP, van der Heide LP. Cell Death Discov. 4:107. doi: 10.1038/s41420-018-0125-7. IF 8.5
19. miR-34b/c enhances mesencephalic dopaminergic neuron differentiation by negatively modulating Wnt signaling (2018) Roberto De Gregorio, Salvatore Pulcrano, Claudia De Sanctis, Floriana Volpicelli, Ezia Guatteo, Lars Von Oerthel, Roberta Esposito1, Rosa Maria Piscitelli, Carla Perrone Capano, Valerio Costa1, Dario Greco, Marten Smidt, Umberto di Porzio, Massimiliano Caiazzo, Nicola Biagio Mercuri, Meng Li and Gian Carlo Bellenchi. Stem Cell Reports 1-, 1-14; IP 7.5
20. Mesman S, Krüse SJ, Smidt MP. (2018) Expression analyzes of early factors in midbrain differentiation programs. Gene Expr Patterns. 27:8-15. doi: 10.1016/j.gep.2017.09.001. Epub 2017
21. Simone Mesman and Marten P. Smidt (2017) Tcf12 Is Involved in Early Cell-Fate Determination and Subset Specification of Midbrain Dopamine Neurons. Front. Mol. Neurosci. 10:353. doi: 10.3389/fnmol.2017.00353 IP 6
22. Smidt MP. (2017) Molecular Programming of Mesodiencephalic Dopaminergic Neuronal Subsets. Front Neuroanat. 2017 Jul 19;11:59. doi: 10.3389/fnana.2017.00059.
23. Kouwenhoven WM, von Oerthel L, Smidt MP (2017) Pitx3 and En1 determine the size and molecular programming of the dopaminergic neuronal pool. PLoS One. 12(8):e0182421. doi: 10.1371/journal.pone.0182421 IF 3
24. Miguel A.P. Oliveira, Rudi Balling, Marten P. Smidt, Ronan M.T. Fleming (2017) Embryonic development of selectively vulnerable neurons in Parkinson’s disease. Nature Partner Journal (NPJ): Parkinson's Disease, Jun 26;3:21. doi: 10.1038/s41531-017-0022-4. IF 8
25. Mesman S, van Hooft JA and Smidt MP (2017) Mest/Peg1 is essential for the development and maintenance of a SNc neuronal subset. Front. Mol. Neurosci. | doi: 10.3389/fnmol.2016.00166 IP 6
26. Paap RH, Oosterbroek S, Wagemans CM, von Oerthel L, Schellevis RD, Vastenhouw-van der Linden AJ, Groot Koerkamp MJ, Hoekman MF, Smidt MP. (2016) FoxO6 affects Plxna4-mediated neuronal migration during mouse cortical development. Proc Natl Acad Sci U S A vol. 113 no. 45, E7087–E7096 IP 10
27. Willemieke M. Kouwenhoven and Marten P. Smidt. (2016) Commentary: engrailed 1 shapes the dopaminergic and serotonergic landscape through proper Iso maintenance and function J. Neurology & Neuromedicin 1(5), 1-3.
28. Kouwenhoven WM, Veenvliet JV, van Hooft JA, van der Heide LP, Smidt MP Engrailed 1 shapes the dopaminergic and serotonergic landscape through proper isthmic organizer maintenance and function. (2016) Biol Open 5(3):279-88. doi: 10.1242/bio.015032. IP 4
29. van der Heide LP, Wijchers PJ, von Oerthel L, Burbach JP, Hoekman MF, Smidt MP. (2015) FoxK2 is required for cellular proliferation and survival. J Cell Physiol. 230(5):1013-23. doi: 10.1002/jcp.24828. IP 5
30. Veenvliet JV, Smidt MP. (2014) Molecular mechanisms of dopaminergic subset specification: fundamental aspects and clinical perspectives. Cell Mol Life Sci. 71(24):4703-27 2014 Jul 27. [Epub ahead of print] IP 6.
31. Inês Chaves, Gijsbertus T.J. van der Horst, Raymond Schellevis, Romana M. Nijman, Marian Groot Koerkamp, Frank C.P. Holstege, Marten P. Smidt and Marco F.M. Hoekman (2014) FoxO3 in control of the hepatic circadian oscillator via direct regulation of Clock. Current Biology 2;24(11):1248-55. doi: 10.1016/j.cub.2014.04.018. IP 11
32. Simone Mesman, Lars von Oerthel and Marten P. Smidt (2014) Mesodiencephalic dopaminergic neuronal differentiation does not involve GLI2A-mediated SHH-signaling and is under the direct influence of canonical WNT signaling. PLosOne May 27;9(5):e97926. doi: 10.1371/journal.pone.0097926. eCollection 2014. IP 4
33. Marlies Oostland, M. Renate Buijink, Guus M. Teunisse, Lars von Oerthel, Marten P. Smidt, Johannes A. van Hooft.(2014) Distinct Temporal Expression of 5-HT1A and 5-HT2A Receptors on Cerebellar Granule Cells in Mice. Cerebellum 13(4):491-500. doi: 10.1007/s12311-014-0565-4. IP 3.
34. Reinhard Roessler, Sebastien A Smallwood, Jesse V Veenvliet, Petros Pechlivanoglou, Suping Peng1, Koushik Chakrabarty, Marian JA Groot-Koerkamp, R. Jeroen Pasterkamp, Evelyn Wesseling, Gavin Kelsel, Erik Boddeke Marten P Smidt and Sjef Copray. (2014) Detailed analysis of the genetic and epigenetic signature of iPS cell-derived mesodiencephalic dopaminergic neurons. Stem Cell Reports 2(4):520-33. doi: 10.1016/j.stemcr.2014.03.001 (Cell press) IP 7.5
35. Smidt MP, van Hooft JA. Subset specification of central serotonergic neurons. (2013) Front Cell Neurosci. 7:200. Review. IP 5
36. Smits SM, von Oerthel L, Hoekstra EJ, Burbach JPH, Smidt MP (2013) Molecular Marker Differences Relate to Developmental Position and Subsets of Mesodiencephalic Dopaminergic Neurons. PLoS ONE 8(10): e76037. doi:10.1371/journal.pone.0076037 IP 4.5
37. Hoekstra EJ, von Oerthel L, van der Heide LP, Kouwenhoven WM, Veenvliet JV, Wever I, Jin YR, Yoon JK, van der Linden AJ, Holstege FC, Groot Koerkamp MJ, Smidt MP. (2013) Lmx1a Encodes a Rostral Set of Mesodiencephalic Dopaminergic Neurons Marked by the Wnt/B-Catenin Signaling Activator R-spondin 2. PLoS One. 2013 Sep 16;8(9):e74049. doi: 10.1371/journal.pone.0074049. IP 4.5
38. Jesse V Veenvliet*, Maria TM Alves dos Santos*, Willemieke M Kouwenhoven, Lars von Oerthel, Jamie L Lim, Annemarie JA van der Linden, Marian JA Groot Koerkamp, Frank CP Holstege and Marten P Smidt (2013) En1 and Pitx3 interplay in dopaminergic subset-specification. Development 140, 3373-3384 (2013) doi:10.1242/dev.094565. *shared first authorship IP7
39. Mareen Engel, Marten P Smidt*, Johannes A Van Hooft* (2013) The serotonin 5-HT3 receptor: a novel neurodevelopmental target. Frontiers in Neuroscience in press. Shared last author, IP 5
40. Lars P. van der Heide and Marten P. Smidt (2013) The BCL2-code to dopaminergic development and Parkinson's disease. Trends Mol Med. 19(4):211-6. IP 11.
41. van Heesbeen HJ, Mesman S, Veenvliet JV, Smidt MP. (2013) Epigenetic mechanisms in the development and maintenance of dopaminergic neurons. Development. 2013 Mar;140(6):1159-69. doi: 10.1242/dev.089359. IP 7.
42. Nott A, Nitarska J, Veenvliet JV, Schacke S, Derijck AA, Sirko P, Muchardt C, Pasterkamp RJ, Smidt MP, Riccio A. (2013) S-nitrosylation of HDAC2 regulates the expression of the chromatin-remodeling factor Brm during radial neuron migration. Proc Natl Acad Sci U S A. 110(8):3113-8. IP 10.
43. Hoekstra EJ, Mesman S, de Munnik WA, Smidt MP. (2013) Lmx1b Is Part of a Transcriptional Complex with PSPC1 and PSF. PLoS One. 2013;8(1):e53122. doi: 10.1371/journal.pone.0053122. Epub 2013 Jan 4. IP 5
44. Hoekstra EJ, von Oerthel L, van der Linden AJ, Smidt MP. (2012) Phox2b influences the development of a caudal dopaminergic subset. PLoS One. 2012;7(12):e52118. doi: 10.1371/journal.pone.0052118. Epub 2012 Dec 14. IP 5
45. Elisa J Hoekstra, Lars von Oerthel, Annemarie J A van der Linden, Raymond D Schellevis, Gerard Scheppink, Frank C.P. Holstege, Marian J Groot-Koerkamp, Lars P van der Heide2, Marten P Smidt (2012) Lmx1a is an activator of Rgs4 and Gbr10 and is responsible for the correct specification of rostral and medial mdDA neurons. Eur J Neurosci. 2012 Oct 29. doi: 10.1111/ejn.12022. IP 3.6
46. Frank Meye, Ruud van Zessen, Marten Smidt, Roger Adan, and Geert Ramakers (2012) Morphine withdrawal enhances constitutive mu-opioid receptor activity in the ventral tegmental area. J Neurosci. 32(46):16120-8. doi: 10.1523/JNEUROSCI.1572-12.2012. IP 8
47. Smidt MP, von Oerthel L, Hoekstra EJ, Schellevis RD, Hoekman MFM (2012) Spatial and Temporal Lineage Analysis of a Pitx3-Driven Cre-Recombinase Knock-In Mouse Model. PLoS ONE 7(8): e42641. doi:10.1371/journal.pone.0042641
48. Koushik Chakrabarty, Lars von oerthel, Anita Hellemons, M. frederic Clotman, Marian J.A. Groot Koerkamp, Frank C.P. Holstege, R. Jeroen Pasterkamp, and Marten P. Smidt (2012) Genome wide expression profiling of the mesodiencephalic region identifies novel factors involved in early and late dopaminergic development. Biology Open 1:693-704.
49. Laura A. Smit-Rigtera, Cornelle W. Noorlander, Lars von Oerthelb, Pascal Chameau, Marten P. Smidt*, Johannes A. van Hooft* (2012) Prenatal fluoxetine exposure induces life-long serotonin 5-HT3 receptor-dependent cortical abnormalities and anxiety-like behaviour Neuropharmacology Volume 62, Issue 2: 865–870 * Shared last author IP 4
50. Frank M.J. Jacobs*, Jesse V. Veenvliet*, Wadia H. Almirza, Elisa Hoekstra, Lars von Oerthel, Annemarie J.A. van der Linden, Roel Neijts, Marian Groot Koerkamp, Dik van Leenen, Frank C.P. Holstege, J. Peter H. Burbach and Marten P. Smidt, Retinoic acid-dependent and -independent gene-regulatory pathways of Pitx3 in meso-diencephalic dopaminergic neurons, Development 2011, 138(23):5213-22. * shared first author. IP 8
51. Alves dos Santos MTM and Smidt MP. En1 and Wnt signaling in midbrain dopaminergic neuronal development. Neural Development 2011, 6:23 doi:10.1186/1749-8104-6-23 IP 3.4
52. Arango C., Gustincich S., Marin O., Parnavelas J. and Smidt M.*, Chapter I: Brain development, plasticity and ageing in health and disease (Section Editor: Monica Di Luca) (Page 770) in; Di Luca M, Baker M, Corradetti R, Kettenmann H, Mendlewicz J, Olesen J, Ragan I, Westphal M. Consensus Document on European Brain Research, Eur. J. Neurosci. 2011 Mar;33(5):768-818 * Theme leader.
53. Rosemann M, Ivashkevich A, Favor J, Dalke C, Hölter SM, Becker L, Rácz I, Bolle I, Klempt M, Rathkolb B, Kalaydjiev S, Adler T, Aguilar A, Hans W, Horsch M, Rozman J, Calzada-Wack J, Kunder S, Naton B, Gailus-Durner V, Fuchs H, Schulz H, Beckers J, Busch DH, Burbach JP, Smidt MP, Quintanilla-Martinez L, Esposito I, Klopstock T, Klingenspor M, Ollert M, Wolf E, Wurst W, Zimmer A, Hrabé de Angelis M, Atkinson M, Heinzmann U, Graw J. Microphthalmia, parkinsonism, and enhanced nociception in Pitx3 (416insG) mice. Mamm Genome. 2010 Feb;21(1-2):13-27.
54. Smidt MP. Specific vulnerability of substantia nigra compacta neurons. J Neural Transm Suppl. 2009;(73):39-47. (review upon invitation) IF 2.4
55. Kolk SM, Gunput RA, Tran TS, van den Heuvel DM, Prasad AA, Hellemons AJ, Adolfs Y, Ginty DD, Kolodkin AL, Burbach JP, Smidt MP, Pasterkamp RJ. Semaphorin 3F is a bifunctional guidance cue for dopaminergic axons and controls their fasciculation, channeling, rostral growth, and intracortical targeting. J Neurosci. 2009 Oct 7;29(40):12542-57. IP 8
56. Smidt MP* and Burbach JPH. Terminal differentiation of mesodiencephalic dopaminergic neurons: the role of Nurr1 and Pitx3. Adv. Exp. Med. Biol. 2009;651:47-57.*corresponding author. (IP 0.67)
57. Development and engineering of dopamine neurons. Preface. Pasterkamp RJ, Smidt MP, Burbach JP. Adv Exp Med Biol. 2009, 651.
58. Smidt MP* and Burbach JPH A passport to neurotransmitter identity. Genome Biology 2009, 10:229. *corresponding author. IP 9. (review upon invitiation)
59. Jacobs FMJ, van der Linden AJA, Wang Y, von Oerthel L, Sul HS, Burbach JPH and Smidt MP. Identification of Dlk1, Ptpru and Klhl1 as novel Nurr1 target genes in meso-diencephalic dopamine neurons. Development 2009 Jul;136(14):2363-73 (selected to feature on the In this Issue page of Issue 136.14). IF 8
60. Jacobs FMJ, van Erp S,van der Linden AJA, von Oerthel L, Burbach JPH and Smidt MP. Pitx3 potentiates Nurr1 in dopamine neuron terminal differentiation through release of SMRT-mediated repression. Development 2009 Feb;136(4):531-40 (selected to feature on the In this Issue page of Issue 136.04) IF 8.
61. Noorlander, CW, Ververs, FFT, Nikkels, PGJ, van Echteld, CJA, Visser, GHA, Smidt, MP. Modulation of serotonin transporter function during fetal development causes dilated heart cardiomyopathy and lifelong behavioral abnormalities. PLoSONE June 23, 2008.
62. Smits SM, Noorlander CW, Kas MJ, Ramakers GM, Smidt MP. Alterations in serotonin signalling are involved in the hyperactivity of Pitx3-deficient mice. Eur J Neurosci. 2008 Jan;27(2):388-95. PMID: 18215235 IP 4.2
63. Vastenhouw B, van der Have F, van der Linden AJ, von Oerthel L, Booij J, Burbach JP, Smidt MP, Beekman FJ. Movies of dopamine transporter occupancy with ultra-high resolution focusing pinhole SPECT. Mol Psychiatry. 2007 Nov;12(11):984-7. Epub 2007 Jul 10. PMID: 17957236 IF 12
64. Kas MJ, van der Linden AJ, Oppelaar H, von Oerthel L, Ramakers GM, Smidt MP. Phenotypic segregation of aphakia and Pitx3-null mutants reveals that Pitx3 deficiency increases consolidation of specific movement components. Behav Brain Res. 2008 Jan 25;186(2):208-14. Epub 2007 Aug 31. PMID: 17919745 IF 2.4
65. Jacobs FM, Smits SM, Noorlander CW, von Oerthel L, van der Linden AJ, Burbach JP, Smidt MP. Retinoic acid counteracts developmental defects in the substantia nigra caused by Pitx3 deficiency. Development. 2007 Jul;134(14):2673-84. PMID: 17592014 IF 8
66. Kramer ER, Aron L, Ramakers GM, Seitz S, Zhuang X, Beyer K, Smidt MP, Klein R. Absence of Ret signaling in mice causes progressive and late degeneration of the nigrostriatal system. PLoS Biol. 2007 Mar;5(3):e39. PMID: 17298183 IF 15
67. Smidt MP*, Burbach JP. How to make a mesodiencephalic dopaminergic neuron. Nat Rev Neurosci. 2007 Jan;8(1):21-32. Review. PMID: 17180160 * corresponding author IF 27
68. Burbach JP, Smidt MP. Molecular programming of stem cells into mesodiencephalic dopaminergic neurons. Trends Neurosci. 2006 Nov;29(11):601-3. Epub 2006 Oct 9. Review. PMID: 17030431 IF 14
69. Smits SM, Smidt MP. The role of Pitx3 in survival of midbrain dopaminergic neurons. J Neural Transm Suppl. 2006;(70):57-60. Review. IF 2.4
70. Sgadò P, Albéri L, Gherbassi D, Galasso SL, Ramakers GM, Alavian KN, Smidt MP, Dyck RH, Simon HH. Slow progressive degeneration of nigral dopaminergic neurons in postnatal Engrailed mutant mice. Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):15242-7. Epub 2006 Oct 2. PMID: 17015829 IF 10
71. van der Heide LP, Ramakers GM, Smidt MP. Insulin signaling in the central nervous system: learning to survive. Prog Neurobiol. 2006 Jul;79(4):205-21. Epub 2006 Aug 17. Review. PMID: 16916571 IF 12
72. de Rover M, Lodder JC, Smidt MP, Brussaard AB. Pitx3 deficiency in mice affects cholinergic modulation of GABAergic synapses in the nucleus accumbens. J Neurophysiol. 2006 Oct;96(4):2034-41. Epub 2006 Jul 12. PMID: 16837663 IF 3.9
73. Jacobs FM, Smits SM, Hornman KJ, Burbach JP, Smidt MP. Strategies to unravel molecular codes essential for the development of meso-diencephalic dopaminergic neurons. J Physiol. 2006 Sep 1;575(Pt 2):397-402. Epub 2006 Jun 29. Review. PMID: 16809365 IF 4.4
74. Wijchers PJ, Burbach JP, Smidt MP. In control of biology: of mice, men and Foxes. Biochem J. 2006 Jul 15;397(2):233-46. Review. PMID: 16792526 IF 29
75. Asbreuk CH, van Doorninck JH, Mansouri A, Smidt MP, Burbach JP. Neurohypophysial dysmorphogenesis in mice lacking the homeobox gene Uncx4.1. J Mol Endocrinol. 2006 Feb;36(1):65-71. PMID: 16461927 IF 5.8
76. Smits SM, Burbach JP, Smidt MP. Developmental origin and fate of meso-diencephalic dopamine neurons. Prog Neurobiol. 2006 Jan;78(1):1-16. Epub 2006 Jan 18. Review. PMID: 16414173 IF 12
77. Wijchers PJ, Hoekman MF, Burbach JP, Smidt MP. Identification of forkhead transcription factors in cortical and dopaminergic areas of the adult murine brain. Brain Res. 2006 Jan 12;1068(1):23-33. Epub 2005 Dec 27. PMID: 16376864 IF 1.7
78. Hoekman MF, Jacobs FM, Smidt MP, Burbach JP. Spatial and temporal expression of FoxO transcription factors in the developing and adult murine brain. Gene Expr Patterns. 2006 Jan;6(2):134-40. Epub 2005 Dec 2. PMID: 16326148 IF2.3
79. Wijchers PJ, Hoekman MF, Burbach JP, Smidt MP. Cloning and analysis of the murine Foxi2 transcription factor. Biochim Biophys Acta. 2005 Nov 10;1731(2):133-8. Epub 2005 Oct 5. PMID: 16289364 IF 2.1
80. Smits SM, van der Nobelen S, Hornman KJ, von Oerthel L, Burbach JP, Smidt MP. Signalling through phospholipase C beta 4 is not essential for midbrain dopaminergic neuron survival. Neuroscience. 2005;136(1):171-9. Epub 2005 Sep 28. PMID: 16198487 IF 3.5
81. Smits SM, Mathon DS, Burbach JP, Ramakers GM, Smidt MP. Molecular and cellular alterations in the Pitx3-deficient midbrain dopaminergic system. Mol Cell Neurosci. 2005 Nov;30(3):352-63. Epub 2005 Sep 6. PMID: 16140547 IF 3.8
82. Kleefstra T, Smidt MP, Banning MJ, Oudakker AR, Van Esch H, de Brouwer AP, Nillesen W, Sistermans EA, Hamel BC, de Bruijn D, Fryns JP, Yntema HG, Brunner HG, de Vries BB, van Bokhoven H. Disruption of the gene Euchromatin Histone Methyl Transferase1 (Eu-HMTase1) is associated with the 9q34 subtelomeric deletion syndrome. J Med Genet. 2005 Apr;42(4):299-306. PMID: 15805155 IF 6.3
83. Burbach JP, Smidt MP Response to Simeone: Coexpression of Pitx3 with tyrosine hydroxylase in midbrain dopaminergic neurons, Trends Neurosci. 2005 28(2), 65-66. IF 14.5
84. Beekman FJ, van der Have F, Vastenhouw B, van der Linden AJ, van Rijk PP, Burbach JP, Smidt MP. U-SPECT-I: a novel system for submillimeter-resolution tomography with radiolabeled molecules in mice. J Nucl Med. 2005 Jul;46(7):1194-200. PMID: 16000289 IF 4.9 Best science paper in J. Nucl. Med 2005 award
85. van der Heide LP, Jacobs FM, Burbach JP, Hoekman MF, Smidt MP. FoxO6 transcriptional activity is regulated by Thr26 and Ser184, independent of nucleo-cytoplasmic shuttling. Biochem J. 2005 Nov 1;391(Pt 3):623-9. PMID: 15987244 IF 4.3
86. van der Heide LP, Smidt MP. Regulation of FoxO activity by CBP/p300-mediated acetylation. Trends Biochem Sci. 2005 Feb;30(2):81-6. PMID: 15691653 IF 14.3
87. Mathon DS, Lesscher HM, Gerrits MA, Kamal A, Pintar JE, Schuller AG, Spruijt BM, Burbach JP, Smidt MP, van Ree JM, Ramakers GM. Increased gabaergic input to ventral tegmental area dopaminergic neurons associated with decreased cocaine reinforcement in mu-opioid receptor knockout mice. Neuroscience. 2005;130(2):359-67. PMID: 15664692 IF 3.5
88. Thuret S, Alavian KN, Gassmann M, Lloyd CK, Smits SM, Smidt MP, Klein R, Dyck RH, Simon HH. The neuregulin receptor, ErbB4, is not required for normal development and adult maintenance of the substantia nigra pars compacta. J Neurochem. 2004 Dec;91(6):1302-11. PMID: 15584907 IF 5
89. Smidt MP*, Smits SM, Burbach JP. Homeobox gene Pitx3 and its role in the development of dopamine neurons of the substantia nigra. Cell Tissue Res. 2004 Oct;318(1):35-43. Epub 2004 Aug 6. Review. PMID: 15300495 *corresponding author IF 2.6
90. Smits SM, Terwisscha van Scheltinga AF, van der Linden AJ, Burbach JP, Smidt MP. Species differences in brain pre-pro-neurotensin/neuromedin N mRNA distribution: the expression pattern in mice resembles more closely that of primates than rats. Brain Res Mol Brain Res. 2004 Jun 18;125(1-2):22-8. PMID: 15193419 IF 2.4
91. Van Der Heide LP, Hoekman MF, Smidt MP. The ins and outs of FoxO shuttling: mechanisms of FoxO translocation and transcriptional regulation. Biochem J. 2004 Jun 1;380(Pt 2):297-309. Review. PMID: 15005655 IF 29.4
92. Smidt MP, Smits SM, Bouwmeester H, Hamers FP, van der Linden AJ, Hellemons AJ, Graw J, Burbach JP. Early developmental failure of substantia nigra dopamine neurons in mice lacking the homeodomain gene Pitx3. Development. 2004 Mar;131(5):1145-55. PMID: 14973278 IF 8
93. Mathon DS, Kamal A, Smidt MP, Ramakers GM. Modulation of cellular activity and synaptic transmission in the ventral tegmental area. Eur J Pharmacol. 2003 Nov 7;480(1-3):97-115. Review. PMID: 14623354 IF 2.3
94. Smidt MP, Smits SM, Burbach JP. Molecular mechanisms underlying midbrain dopamine neuron development and function. Eur J Pharmacol. 2003 Nov 7;480(1-3):75-88. Review. PMID: 14623352 IF 2.3
95. Smits SM, Ponnio T, Conneely OM, Burbach JP, Smidt MP. Involvement of Nurr1 in specifying the neurotransmitter identity of ventral midbrain dopaminergic neurons. Eur J Neurosci. 2003 Oct;18(7):1731-8. PMID: 14622207 IF 4.2
96. Kromkamp M, Uylings HB, Smidt MP, Hellemons AJ, Burbach JP, Kahn RS. Decreased thalamic expression of the homeobox gene DLX1 in psychosis. Arch Gen Psychiatry. 2003 Sep;60(9):869-74. PMID: 12963668 IF 12
97. Vogelaar CF, Smits SM, Brakkee JH, Gispen WH, Smidt MP, Schrama LH, Hoekman MFM, Burbach JP Homeobox gene repertoire in adult rat dorsal root ganglia. Neurosci. Res. Commun. 2003;32, 49-59. IF 0.3
98. Jacobs FM, van der Heide LP, Wijchers PJ, Burbach JP, Hoekman MF, Smidt MP. FoxO6, a novel member of the FoxO class of transcription factors with distinct shuttling dynamics. J Biol Chem. 2003 Sep 19;278(38):35959-67. Epub 2003 Jul 11. PMID: 12857750 IF 8
99. Burbach JP, Smits S, Smidt MP. Transcription factors in the development of midbrain dopamine neurons. Ann N Y Acad Sci. 2003 Jun;991:61-8. Review. PMID: 12846974 IF 1.5
100. Asbreuk CH, Vogelaar CF, Hellemons A, Smidt MP, Burbach JP. CNS expression pattern of Lmx1b and coexpression with ptx genes suggest functional cooperativity in the development of forebrain motor control systems. Mol Cell Neurosci. 2002 Nov;21(3):410-20. PMID: 12498783 IF 4.5
101. Asbreuk CH, van Schaick HS, Cox JJ, Smidt MP, Burbach JP. Survey for paired-like homeodomain gene expression in the hypothalamus: restricted expression patterns of Rx, Alx4 and goosecoid. Neuroscience. 2002;114(4):883-9. PMID: 12379244 IF 3.5
102. Asbreuk CH, van Schaick HS, Cox JJ, Kromkamp M, Smidt MP, Burbach JP. The homeobox genes Lhx7 and Gbx1 are expressed in the basal forebrain cholinergic system. Neuroscience. 2002;109(2):287-98. PMID: 11801365 IF 3.5
103. Smidt MP, Cox JJ, van Schaick HS, Coolen M, Schepers J, van der Kleij AM, Burbach JP. Analysis of three Ptx2 splice variants on transcriptional activity and differential expression pattern in the brain. J Neurochem. 2000 Nov;75(5):1818-25. PMID: 11032870 IF 5
104. Cazorla P, Smidt MP, O'Malley KL, Burbach JP. A response element for the homeodomain transcription factor Ptx3 in the tyrosine hydroxylase gene promoter. J Neurochem. 2000 May;74(5):1829-37. PMID: 10800925 IF 5
105. Smidt MP, Asbreuk CH, Cox JJ, Chen H, Johnson RL, Burbach JP. A second independent pathway for development of mesencephalic dopaminergic neurons requires Lmx1b. Nat Neurosci. 2000 Apr;3(4):337-41. PMID: 10725922 IF 15
106. Burbach JP, van Schaick H, Lopes da Silva S, Asbreuk CH, Smidt MP. Hypothalamic transcription factors and the regulation of the hypothalamo-neurohypophysial system. Adv Exp Med Biol. 1998;449:29-37. Review. PMID: 10026783
107. Smidt MP, Snippe L, van Keulen G, Ab G. The bZip transcription factor vitellogenin-binding protein is post transcriptional down regulated in chicken liver. Eur J Biochem. 1998 Aug 15;256(1):106-11. PMID: 9746352 IF 3
108. Saucedo-Cardenas O, Quintana-Hau JD, Le WD, Smidt MP, Cox JJ, De Mayo F, Burbach JP, Conneely OM. Nurr1 is essential for the induction of the dopaminergic phenotype and the survival of ventral mesencephalic late dopaminergic precursor neurons. Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):4013-8. PMID: 9520484 IF 9
109. Smidt MP, van Schaick HS, Lanctôt C, Tremblay JJ, Cox JJ, van der Kleij AA, Wolterink G, Drouin J, Burbach JP. A homeodomain gene Ptx3 has highly restricted brain expression in mesencephalic dopaminergic neurons. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13305-10. PMID: 9371841 IF 9
110. van Schaick HS*, Smidt MP*, Rovescalli AC, Luijten M, van der Kleij AA, Asoh S, Kozak CA, Nirenberg M, Burbach JP. Homeobox gene Prx3 expression in rodent brain and extraneural tissues. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):12993-8. PMID: 9371788 *shared first author IF 9
111. Smidt MP, Russchen B, Snippe L, Wijnholds J, Ab G. Cloning and characterisation of a nuclear, site specific ssDNA binding protein. Nucleic Acids Res. 1995 Jul 11;23(13):2389-95. PMID: 7630716 IF 7
112. Smidt MP, Wijnholds J, Snippe L, van Keulen G, Ab G. Binding of a bZip protein to the estrogen-inducible apoVLDL II promoter. Biochim Biophys Acta. 1994 Sep 13;1219(1):115-20. PMID: 8086448 IF 4
113. van der Ploeg J, Smidt MP, Landa AS, Janssen DB. Identification of Chloroacetaldehyde Dehydrogenase Involved in 1,2-Dichloroethane Degradation. Appl Environ Microbiol. 1994 May;60(5):1599-1605. PMID: 16349259 IF 4
114. Beintema JJ, Stam WT, Hazes B, Smidt MP. Evolution of arthropod hemocyanins and insect storage proteins (hexamerins). Mol Biol Evol. 1994 May;11(3):493-503. PMID: 8015442 IF 5.3
115. Van Rijssel M, Smidt MP, Van Kouwen G, Hansen TA. Involvement of an Intracellular Oligogalacturonate Hydrolase in Metabolism of Pectin by Clostridium thermosaccharolyticum. Appl Environ Microbiol. 1993 Mar;59(3):837-842. PMID: 16348892 IF 4

The molecular programming of neuronal systems team

Marten Smidt

Principle investigator

Simone Mesman

Assistant professor

Molecular coding of neurodevelopment

Matthijs Lingl

PhD student.

Epigenetics of neuronal development 

Martijn Bast 

Research technician.

Monica Anderson Berdal

Research Technician

Lars van der Heide

Associate Professor

Marco Hoekman

Assistent professor

Anke Dijkstra.

Assistent Professor

It is my pleasure to announce the construction of a new drug development company : Macrobianbiotech.com

This company is founded by Dr. Lars P. van de Heide and Prof. Dr. Marten P. Smidt with the University of Amsterdam (Holding).

Education

Involved courses at the UvA: 

• Ontwikkelingsbiologie, Ontwikkelingspsychologie & Taal, (Bachelor PB, Year 2)
• Molecular biology of the cell ( Bachelor PB, year 1)
• Techniques in Molecular biology (Bachelor PB, year2/3)
• Introduction in the Neurobiology, (Bachelor medical sciences, year 3)
• Developed and certified an Art. 9, WOD course scheduled for june 2014 onwards; Provides the formal certification (Art. 9, WOD at 2014) for animal experimentation in the EU.
• Master program Molecular NeuroScience (MNS)/NeuroDevelopment
• Tesla supervisor

Internship possibilities

Please contact marten smidt, or other lab members by email for new internship possibilities; see the labmembers tab.

Managment

Founder/CEO of Macrobian-biotech (2017-current).

Director of the Swammerdam Institute of Life Sciences, UvA (2015-2021).

Chairman of the exam committee of the Bachelor Psychobiology (2012-2016).

Chairman/director of the Neurobiology cluster of the master Biomedical sciences (2012-2016).

Chairman of the VIDI-ALW committee (2011-2014).

Member of the NWO-ENW VICI committee (2018)

Co-director of Amsterdam Neuroscience (2022-)

EU-Programs

Marten Smidt has been a coordinator of the MdDAneurodev program (FP7 collaborative project; 222999) "Molecular coding and subset specification of dopamine neurons generating the meso-limbic andf nigrostriatal system"

The project was evaluated as excellent en resulted in many publications on the topic by the respective consortium members. The project was finalized at the end of 2012.

Consortium partners:

Prof. W. Wurst; Prof. W. Driever; Prof. J Pasterkamp; Prof. O. Marin; Prof T. Perlmann; Prof. A. Prochiantz and Prof A. Simeone.

**Please see the downloads area for the eu published report. 

National growth fund.

Coordinator of the Holomicrobioom initiative, which was funded (reservation of 200 million euro) in july 2023.