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NL

dr. S. (Silke) Allmann

Faculty of Science
Swammerdam Institute for Life Sciences

Visiting address
  • Science Park 904
  • Room number: C2.208
Postal address
  • Postbus 1210
    1000 BE Amsterdam
Contact details
  • Profile

     

    RESEARCH INTERESTS

    Plant leaves can emit large amounts of volatiles into the air. When attacked by insects, the composition of these blends changes markedly. It is well known that these changes affect not only the behavior of insects interacting with the plant but also the metabolism of the plant itself as well as its nearby competitors. However, how plants perceive these volatiles and generate a functional response is not known.

    My research activities have been dedicated to a group of plant volatiles emitted the earliest upon herbivory, the so-called green leaf volatiles (GLVs). We discovered a class of enzymes, present in plants and insects, that profoundly affect ecological interactions by converting the highly abundant GLV Z-3-hexenal into E-2-hexenal. These two compounds, as well as their derivatives, among which Z-3- and E-2-hexenyl acetate, have distinct effects on the behavior of herbivorous and predacious insects as well as on the metabolism of plants.

    1) (3Z):(2E)-hexenal isomerases

    Tobacco hornworm (Manduca sexta)

    During my PhD I investigated how the GLVs of wild tobacco (Nicotiana attenuata) modulate the behaviour of herbivorous and carnivorous arthropods. We discovered two important new facts. Firstly we found that especially the ratio between Z-3- and E-2-isomers determines how insects respond. Secondly, we discovered that the conversion of Z-3-hexenal to E-2-hexenal (thereby changing their ratio) was not mediated by the plant (as was always assumed) but by the feeding caterpillar (Manduca sexta). This unexpected observation called for a revised paradigm on the adaptiveness of induced volatiles in plant-herbivore interactions. Hence we continued with elucidating the nature and occurrence of this puzzling trait. Recently we have identified the caterpillar enzyme responsible for this phenomenon and characterized it. Interestingly, also some plant species (but not wild tobacco) are capable of performing the same conversion. We are now interested in understanding the importance of hexenal isomerisation on the development of insect larvae and host-plant selection by adults.

    2) Volatile perception

    Arabidopsis

    Plants display distinct reproducible responses after perception of GLVs. These responses include the transcriptional upregulation of specific subsets of defense-related genes and accumulation of downstream metabolites. Besides immediate activation, plant defenses can also be primed by GLV exposure: this enables a plant to upregulate defenses more rapidly and/or more effectively at the actual moment of attack. Our knowledge of the molecular basis of odour perception by insects has grown enormously in recent years. However, far less progress has been made in our understanding of odour perception in plants. Previous work by my colleague Rob Schuurink has led to the identification of the first Arabidopsis mutants that are resistant to E-2-hexenal (her mutants) and they have mapped the her1 mutation to discover a role for GABA in this process as well as in the defense against Pseudomonas. They also mapped and cloned the her2 mutant and determined that several WRKY transcription factors may regulate the E-2-hexenal response in Arabidopsis. Moreover, in the Arabidopsis-Pseudomonas interaction, the microbe seems to hijack the C6 volatiles to enhance jasmonate biosynthesis and susceptibility. The research on E-2-hexenal signaling will be continued by my group using transcriptomics and proteomics approaches.

    Inhibition of root growth in Arabidopsis seedlings by E-2-hexenal exposure: Arabidopsis seedlings normally respond to E-2-hexenal exposure with the inhibition of root growth. This root inhibition assay has been used to identify mutants which are unable to respond to E-2-hexenal.

     

     

    PLANT PHYSIOLOGY

    I work in the Plant Physiology department headed by Prof. Dr. Michel Haring. Other staff members are:

    Dr. Robert Schuurink (Perception and signaling in plant-insect interactions)

    Dr. Petra Bleeker (Natural variation in specialised metabolites against pests)

  • Lab members

     

    THE GROUP

    Silke Allmann (group leader)
    Martha van Os (PhD - ERC starting grant)
    Juliette Silven (technician - ERC starting grant)
    Abigail Brock (lab assistant)

     

    FORMER LAB MEMBERS

    Maurice Heilijgers (lab assistant)
  • Publications

    2018

    • Ameye, M., Allmann, S., Verwaeren, J., Smagghe, G., Haesaert, G., Schuurink, R. C., & Audenaert, K. (2018). Green leaf volatile production by plants: a meta-analysis. New Phytologist, 220(3), 666-683. https://doi.org/10.1111/nph.14671 [details]

    2017

    2015

    • Mirabella, R., Rauwerda, H., Allmann, S., Scala, A., Spyropoulou, E. A., de Vries, M., ... Schuurink, R. C. (2015). WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal in Arabidopsis. Plant Journal, 83(6), 1082-1096. https://doi.org/10.1111/tpj.12953 [details]
    • Schuman, M. C., Allmann, S., & Baldwin, I. T. (2015). Plant defense phenotypes determine the consequences of volatile emission for individuals and neighbors. eLife, 2015(4), 04490. https://doi.org/10.7554/eLife.04490 [details]

    2013

    • Allmann, S., Späthe, A., Bisch-Knaden, S., Kallenbach, M., Reinecke, A., Sachse, S., ... Hansson, B. S. (2013). Feeding-induced rearrangement of green leaf volatiles reduces moth oviposition. eLife, 2, e00421. https://doi.org/10.7554/eLife.00421 [details]
    • Scala, A., Allmann, S., Mirabella, R., Haring, M. A., & Schuurink, R. C. (2013). Green leaf volatiles: a plant's multifunctional weapon against herbivores and pathogens. International Journal of Molecular Sciences, 14(9), 17781-177811. https://doi.org/10.3390/ijms140917781 [details]

    2012

    • Alba, J. M., Allmann, S., Glas, J. J., Schimmel, B. C. J., Spyropoulou, E. A., Stoops, M., ... Kant, M. R. (2012). Induction and suppression of herbivore-induced indirect defenses. In G. Witzany, & F. Baluška (Eds.), Biocommunication of plants (pp. 197-212). (Signaling and communication in plants; No. 14). Berlin/Heidelberg: Springer. https://doi.org/10.1007/978-3-642-23524-5_11 [details]

    2011

    • Diezel, C., Allmann, S., & Baldwin, I. T. (2011). Mechanisms of optimal defense patterns in Nicotiana attenuata: flowering attenuates herbivory-elicited ethylene and jasmonate signaling. Journal of Integrative Plant Biology, 53(12), 971-983. https://doi.org/10.1111/j.1744-7909.2011.01086.x [details]
    • Kallenbach, M., Gilardoni, P. A., Allmann, S., Baldwin, I. T., & Bonaventure, G. (2011). C12 derivatives of the hydroperoxide lyase pathway are produced by product recycling through lipoxygenase-2 in Nicotiana attenuata leaves. New Phytologist, 191(4), 1054-1068. https://doi.org/10.1111/j.1469-8137.2011.03767.x [details]

    2010

    2008

    • Wang, L., Allmann, S., Wu, J., & Baldwin, I. T. (2008). Comparisons of LIPOXYGENASE3- and JASMONATE-RESISTANT4/6-silenced plants reveal that jasmonic acid and jasmonic acid-amino acid conjugates play different roles in herbivore resistance of Nicotiana attenuate[W][OA]. Plant Physiology, 146(3), 904-915. https://doi.org/10.1104/pp.107.109264

    Media appearance

    • Allmann, S. (22-10-2014). Kunnen planten met elkaar praten? [Television] Evangelische Omroep. Kunnen planten met elkaar praten?.
    This list of publications is extracted from the UvA-Current Research Information System. Questions? Ask the library or the Pure staff of your faculty / institute. Log in to Pure to edit your publications. Log in to Personal Page Publication Selection tool to manage the visibility of your publications on this list.
  • Ancillary activities
    • No ancillary activities