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Dr. J.E. (Jurgen) Seppen

Faculty of Science

Visiting address
  • Science Park 904
Postal address
  • Postbus 94214
    1090 GE Amsterdam
Contact details
  • Publications



    • Koopman, N., Katsavelis, D., Ten Hove, A. S., Brul, S., de Jonge, W. J., & Seppen, J. (2021). The multifaceted role of serotonin in intestinal homeostasis. International Journal of Molecular Sciences, 22(17), [9487]. [details]
    • Ten Hove, A. S., Seppen, J., & de Jonge, W. J. (2021). Neuronal innervation of the intestinal crypt. American Journal of Physiology - Gastrointestinal and Liver Physiology, 320(2), G193-G205.


    • Diederen, K., Li, J. V., Donachie, G. E., de Meij, T. G., de Waart, D. R., Hakvoort, T. B. M., Kindermann, A., Wagner, J., Auyeung, V., te Velde, A. A., Heinsbroek, S. E. M., Benninga, M. A., Kinross, J., Walker, A. W., de Jonge, W. J., & Seppen, J. (2020). Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn’s disease. Scientific Reports, 10(1), [18879].
    • van Thiel, I. A. M., Botschuijver, S., de Jonge, W. J., & Seppen, J. (2020). Painful interactions: Microbial compounds and visceral pain. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1866(1), [165534].


    • Végh, A. M. D., Dénise den Haan, A., Ortega, L. C., Verkerk, A. O., Sluijter, J. P. G., Bakker, D., Van Amersfoorth, S., Van Veen, T. A. B., Klerk, M., Seppen, J., De Bakker, J. M. T., Christoffels, V. M., Geerts, D., Goumans, M. J. T. H., Tan, H. L., & Boink, G. J. J. (2019). Cardiomyocyte progenitor cells as a functional gene delivery vehicle for long-term biological pacing. Molecules, 24(1), [181].
    • Willemze, R. A., Brinkman, D. J., Welting, O., Van Hamersveld, P. H. P., Verseijden, C., Luyer, M. D., Wildenberg, M. E., Seppen, J., & de Jonge, W. J. (2019). Acetylcholine-producing T cells augment innate immune-driven colitis but are redundant in T cell-driven colitis. American Journal of Physiology - Gastrointestinal and Liver Physiology, 317(5), G557-G568.
    • Willemze, R. A., Welting, O., van Hamersveld, P., Verseijden, C., Nijhuis, L. E., Hilbers, F. W., Meijer, S. L., Heesters, B. A., Folgering, J. H. A., Darwinkel, H., Blancou, P., Vervoordeldonk, M. J., Seppen, J., Heinsbroek, S. E. M., & de Jonge, W. J. (2019). Loss of intestinal sympathetic innervation elicits an innate immune driven colitis. Molecular Medicine, 25(1), [1].


    • Botschuijver, S., Welting, O., Levin, E., Maria-Ferreira, D., Koch, E., Montijn, R. C., Seppen, J., Hakvoort, T. B. M., Schuren, F. H. J., de Jonge, W. J., & van den Wijngaard, R. M. (2018). Reversal of visceral hypersensitivity in rat by Menthacarin®, a proprietary combination of essential oils from peppermint and caraway, coincides with mycobiome modulation. Neurogastroenterology and motility, 30(6), [e13299].
    • Willemze, R. A., Welting, O., van Hamersveld, H. P., Meijer, S. L., Folgering, J. H. A., Darwinkel, H., Witherington, J., Sridhar, A., Vervoordeldonk, M. J., Seppen, J., & de Jonge, W. J. (2018). Neuronal control of experimental colitis occurs via sympathetic intestinal innervation. Neurogastroenterology and motility, 30(3), [e13163].


    • Botschuijver, S., Roeselers, G., Levin, E., Jonkers, D. M., Welting, O., Heinsbroek, S., de Weerd, H. H., Boekhout, T., Fornai, M., Masclee, A. A., Schuren, F. H. J., de Jonge, W. J., Seppen, J., & van den Wijngaard, R. M. (2017). Intestinal Fungal Dysbiosis Associated With Visceral Hypersensitivity in Patients With Irritable Bowel Syndrome and Rats. Gastroenterology, 153(4), 1026-1039. [details]
    • Van Dijk, R., Aronson, S. J., De Waart, D. R., Van De Graaf, S. F., Duijst, S., Seppen, J., Elferink, R. O., Beuers, U., & Bosma, P. J. (2017). Biliverdin Reductase inhibitors did not improve severe unconjugated hyperbilirubinemia in vivo. Scientific Reports, 7(1), [1646].


    • Dhawan, S., de Palma, G., Willemze, R. A., Hilbers, F. W., Verseijden, C., Luyer, M. D., Nuding, S., Wehkamp, J., Souwer, Y., de Jong, E. C., Seppen, J., van den Wijngaard, R. M., Wehner, S., Verdu, E., Bercik, P., & de Jonge, W. J. (2016). Acetylcholine-producing T cells in the intestine regulate antimicrobial peptide expression and microbial diversity. American Journal of Physiology - Gastrointestinal and Liver Physiology, 311(5), G920-G933.
    • Schilderink, R., Verseijden, C., Seppen, J., Muncan, V., van den Brink, G. R., Lambers, T. T., van Tol, E. A., & de Jonge, W. J. (2016). The SCFA butyrate stimulates the epithelial production of retinoic acid via inhibition of epithelial HDAC. American Journal of Physiology - Gastrointestinal and Liver Physiology, 310(11), G1138-G1146.


    • Kingma, S. D. K., Wagemans, T., IJlst, L., Seppen, J., Gijbels, M. J. J., Wijburg, F. A., & van Vlies, N. (2015). Adverse effects of genistein in a mucopolysaccharidosis type I mouse model. In JIMD Reports (pp. 77-83). (JIMD Reports; Vol. 23). Springer.


    • El Filali, E., Duijst, S., Hiralall, J. K., Legrand, N., van Gulik, T., Hoekstra, R., & Seppen, J. (2014). Human fetal liver cells for regulated ex vivo erythropoietin gene therapy. Molecular Therapy - Methods and Clinical Development, 1, 14003.
    • Van Wenum, M., Chamuleau, R. A., Van Gulik, T. M., Siliakus, A., Seppen, J., & Hoekstra, R. (2014). Bioartificial livers in vitro and in vivo: Tailoring biocomponents to the expanding variety of applications. Expert Opinion on Biological Therapy, 14(12), 1745-1760.


    • Abel, T., Filali, E. E., Waern, J., Schneider, I. C., Yuan, Q., Münch, R. C., Hick, M., Warnecke, G., Madrahimov, N., Kontermann, R. E., Schuttrumpf, J., Müller, U. C., Seppen, J., Ott, M., & Buchholz, C. J. (2013). Specific gene delivery to liver sinusoidal & artery endothelial cells. Blood, 122(12), 2030-2038.
    • Filali, E. E., Hiralall, J. K., van Veen, H. A., Stolz, D. B., & Seppen, J. (2013). Human liver endothelial cells, but not macrovascular or microvascular endothelial cells, engraft in the mouse liver. Cell transplantation, 22(10), 1801-1811.
    • Hoekstra, R., Nibourg, G. A. A., Van Der Hoeven, T. V., Plomer, G., Seppen, J., Ackermans, M. T., Camus, S., Kulik, W., Van Gulik, T. M., Oude Elferink, R. P., & Chamuleau, R. A. F. M. (2013). Phase 1 and phase 2 drug metabolism and bile acid production of HepaRG cells in a bioartificial liver in absence of dimethyl sulfoxide. Drug Metabolism and Disposition, 41(3), 562-567.
    • Seppen, J., & Bruzzone, L. (2013). Lynch syndrome: The patients perspective. Familial Cancer, 12(2), 341-345.


    • Bakker, M. L., Boink, G. J. J., Boukens, B. J., Verkerk, A. O., Van Den Boogaard, M., Den Haan, A. D., Hoogaars, W. M. H., Buermans, H. P., De Bakker, J. M. T., Seppen, J., Tan, H. L., Moorman, A. F. M., T Hoen, P. A. C., & Christoffels, V. M. (2012). T-box transcription factor TBX3 reprogrammes mature cardiac myocytes into pacemaker-like cells. Cardiovascular research, 94(3), 439-449.
    • Falix, F. A., Aronson, D. C., Lamers, W. H., Hiralall, J. K., & Seppen, J. (2012). DLK1, a serum marker for hepatoblastoma in young infants. Pediatric Blood and Cancer, 59(4), 743-745.
    • Folmer, D. E., Mok, K. S., de Wee, S. W., Duijst, S., Hiralall, J. K., Seppen, J., Oude Elferink, R. P. J., & Paulusma, C. C. (2012). Cellular Localization and Biochemical Analysis of Mammalian CDC50A, a Glycosylated β-subunit for P4 ATPases. Journal of Histochemistry and Cytochemistry, 60(3), 205-218.
    • Seppen, J. (2012). A diet containing the soy phytoestrogen genistein causes infertility in female rats partially deficient in UDP glucuronyltransferase. Toxicology and Applied Pharmacology, 264(3), 335-342.
    • Seppen, J., & Bosma, P. (2012). Bilirubin, the gold within. Circulation, 126(22), 2547-2549.


    • Groen, A., Romero, M. R., Kunne, C., Hoosdally, S. J., Dixon, P. H., Wooding, C., Williamson, C., Seppen, J., Van Den Oever, K., Mok, K. S., Paulusma, C. C., Linton, K. J., & Oude Elferink, R. P. J. (2011). Complementary functions of the flippase ATP8B1 and the floppase ABCB4 in maintaining canalicular membrane integrity. Gastroenterology, 141(5), 1927-1937.e4.


    • Centlivre, M., Zhou, X., Pouw, S. M., Weijer, K., Kleibeuker, W., Das, A. T., Blom, B., Seppen, J., Berkhout, B., & Legrand, N. (2010). Autoregulatory lentiviral vectors allow multiple cycles of doxycycline-inducible gene expression in human hematopoietic cells in vivo. Gene Therapy, 17(1), 14-25.
    • Markusic, D. M., de Waart, D. R., & Seppen, J. (2010). Separating lentiviral vector injection and induction of gene expression in time, does not prevent an immune response to rtTA in rats. PLoS ONE, 5(4), e9974. [details]
    • Markusic, D., & Seppen, J. (2010). Doxycycline regulated lentiviral vectors. Methods in Molecular Biology, 614, 69-76.
    • May, T., Butueva, M., Bantner, S., Markusic, D., Seppen, J., MacLeod, R. A. F., Weich, H., Hauser, H., & Wirth, D. (2010). Synthetic gene regulation circuits for control of cell expansion. Tissue Engineering - Part A, 16(2), 441-452.
    • Van Hooft, J., Elferink, R. O., & Seppen, J. (2010). Gulzigheid en boete: Laxatiekuren en 'leverstenen'. Nederlands Tijdschrift voor Geneeskunde, 154(51-52), 2391-2393.


    • Deurholt, T., van Til, N. P., Chhatta, A. A., ten Bloemendaal, L., Schwartlander, R., Payne, C., Plevris, J. N., Sauer, I. M., Chamuleau, R. A. F. M., Oude Elferink, R. P. J., Seppen, J., & Hoekstra, R. (2009). Novel immortalized human fetal liver cell line, cBAL111, has the potential to differentiate into functional hepatocytes. BMC Biotechnology, 9, [89]. [details]
    • Markusic, D. M., van Til, N. P., Hiralall, J. K., Oude Elferink, R. P. J., & Seppen, J. (2009). Reduction of liver macrophage transduction by pseudotyping lentiviral vectors with a fusion envelope from Autographa californica GP64 and Sendai virus F2 domain. BMC Biotechnology, 9(1), 85. [details]
    • Seppen, J., Filali, E. E., & Elferink, R. O. (2009). Small animal models of hepatocyte transplantation. In Hepatocyte Transplantation: Methods and Protocols (pp. 75-82). (Methods in Molecular Biology; Vol. 481). Humana Press.


    • Boink, G. J. J., Verkerk, A. O., van Amersfoorth, S. C. M., Tasseron, S. J., van der Rijt, R., Bakker, D., Linnenbank, A. C., van der Meulen, J., de Bakker, J. M. T., Seppen, J., & Tan, H. L. (2008). Engineering physiologically controlled pacemaker cells with lentiviral HCN4 gene transfer. Journal of Gene Medicine, 10(5), 487-497.
    • van Til, N. P., Heutinck, K. M., van der Rijt, R., Paulusma, C. C., van Wijland, M., Markusic, D. M., Oude Elferink, R. P. J., & Seppen, J. (2008). Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity. Retrovirology, 5, [14].


    • Boink, G. J. J., Seppen, J., De Bakker, J. M. T., & Tan, H. L. (2007). Biological pacing by gene and cell therapy. Netherlands Heart Journal, 15(9), 318-322.
    • Boink, G. J. J., Seppen, J., de Bakker, J. M. T., & Tan, H. L. (2007). Gene therapy to create biological pacemakers. Medical and Biological Engineering and Computing, 45(2), 167-176.
    • Gierman, H. J., Indemans, M. H., Koster, J., Goetze, S., Seppen, J., Geerts, D., van Driel, R., & Versteeg, R. (2007). Domain-wide regulation of gene expression in the human genome. Genome Research, 17(9), 1286-1295. [details]
    • Markusic, D. M., Kanitz, A., Oude-Elferink, R. P. J., & Seppen, J. (2007). Preferential gene transfer of lentiviral vectors to liver-derived cells, using a hepatitis B peptide displayed on GP64. Human Gene Therapy, 18(7), 673-679.
    • Vrins, C., Vink, E., Vandenberghe, K. E., Frijters, R., Seppen, J., & Groen, A. K. (2007). The sterol transporting heterodimer ABCG5/ABCG8 requires bile salts to mediate cholesterol efflux. FEBS Letters, 581(24), 4616-4620.


    • Bonta, P. I., Van Tiel, C. M., Vos, M., Pols, T. W. H., Van Thienen, J. V., Ferreira, V., Arkenbout, E. K., Seppen, J., Spek, C. A., Van Der Poll, T., Pannekoek, H., & De Vries, C. J. M. (2006). Nuclear receptors Nur77, Nurr1, and NOR-1 expressed in atherosclerotic lesion macrophages reduce lipid loading and inflammatory responses. Arteriosclerosis, Thrombosis, and Vascular Biology, 26(10), 2288-2294.
    • Deurholt, T., Ten Bloemendaal, L., Chhatta, A. A., Van Wijk, A. C. W. A., Weijer, K., Seppen, J., Oude Elferink, R. P. J., Chamuleau, R. A. F. M., & Hoekstra, R. (2006). In vitro functionality of human fetal liver cells and clonal derivatives under proliferative conditions. Cell transplantation, 15(8-9), 811-822.
    • Seppen, J., Bakker, C., de Jong, B., Kunne, C., van den Oever, K., Vandenberghe, K., de Waart, R., Twisk, J., & Bosma, P. (2006). Adeno-associated Virus Vector Serotypes Mediate Sustained Correction of Bilirubin UDP Glucuronosyltransferase Deficiency in Rats. Molecular Therapy, 13(6), 1085-1092.
    • Seppen, J., van Til, N. P., van der Rijt, R., Hiralall, J. K., Kunne, C., & Oude Elferink, R. P. J. (2006). Immune response to lentiviral bilirubin UDP-glucuronosyltransferase gene transfer in fetal and neonatal rats. Gene Therapy, 13(8), 672-677.


    • Barry, S., Brzezinski, M., Yanay, O., Seppen, J. E., & Osborne, W. R. A. (2005). Sustained elevation of neutrophils in rats induced by lentivirus-mediated G-CSF delivery. Journal of Gene Medicine, 7(12), 1510-1516.
    • Bot, I., van Eck, M., van Santbrink, P. J., Groot, P. H. E., Hildebrand, R. B., Seppen, J., van Berkel, T. J. C., & Biessen, E. A. L. (2005). Lentiviral shRNA silencing of murine bone marrow cell CCR2 leads to persistent knockdown of CCR2 function in vivo. Blood, 106(4), 1147-1153. [details]
    • Dekker, R. J., Van Thienen, J. V., Rohlena, J., De Jager, S. C., Elderkamp, Y. W., Seppen, J., De Vries, C. J. M., Biessen, E. A. L., Van Berkel, T. J. C., Pannekoek, H., & Horrevoets, A. J. G. (2005). Endothelial KLF2 links local arterial shear stress levels to the expression of vascular tone-regulating genes. American Journal of Pathology, 167(2), 609-618.
    • Markusic, D., Oude-Elferink, R., Das, A. T., Berkhout, B., & Seppen, J. (2005). Comparison of single regulated lentiviral vectors with rtTA expression driven by an autoregulatory loop or a constitutive promoter. Nucleic Acids Research, 33(6), [e63]. [details]
    • van Til, N. P., Markusic, D. M., van der Rijt, R., Kunne, C., Hiralall, J. K., Vreeling, H., Frederiks, W. M., Oude-Elferink, R. P. J., & Seppen, J. (2005). Kupffer cells and not liver sinusoidal endothelial cells prevent lentiviral transduction of hepatocytes. Molecular Therapy, 11(1), 26-34.


    • Carlotti, F., Bazuine, M., Kekarainen, T., Seppen, J., Pognonec, P., Maassen, J. A., & Hoeben, R. C. (2004). Lentiviral vectors efficiently transduce quiescent mature 3T3-L1 adipocytes. Molecular Therapy, 9(2), 209-217.


    • Seppen, J., van der Rijt, R., Looije, N., van Til, N. P., Lamers, W. H., & Oude Elferink, R. P. J. (2003). Long-term correction of bilirubin UDPglucuronyltransferase deficiency in rats by in utero lentiviral gene transfer. Molecular Therapy, 8(4), 593-599.


    • Seppen, J., Rijnberg, M., Cooreman, M. P., & Oude Elferink, R. P. J. (2002). Lentiviral vectors for efficient transduction of isolated primary quiescent hepatocytes. Journal of hepatology, 36(4), 459-465.
    • Weijer, K., Uittenbogaart, C. H., Voordouw, A., Couwenberg, F., Seppen, J., Blom, B., Vyth-Dreese, F. A., & Spits, H. (2002). Intrathymic and extrathymic development of human plasmacytoid dendritic cell precursors in vivo. Blood, 99(8), 2752-2759.


    • Barry, S. C., Harder, B., Brzezinski, M., Flint, L. Y., Seppen, J., & Osborne, W. R. A. (2001). Lentivirus vectors encoding both central polypurine tract and posttranscriptional regulatory element provide enhanced transduction and transgene expression. Human Gene Therapy, 12(9), 1103-1108.
    • Seppen, J., Barry, S. C., Harder, B., & Osborne, W. R. A. (2001). Lentivirus administration to rat muscle provides efficient sustained expression of erythropoietin. Blood, 98(3), 594-596. [details]


    • Barry, S. C., Seppen, J., Ramesh, N., Foster, J. L., Seyama, K., Ochs, H. D., Garcia, J. V., & Osborne, W. R. A. (2000). Lentiviral and murine retroviral transduction of T cells for expression of human CD40 ligand. Human Gene Therapy, 11(2), 323-332.
    • Seppen, J., Barry, S. C., Klinkspoor, J. H., Katen, L. J., Lee, S. P., Garcia, J. V., & Osborne, W. R. A. (2000). Apical gene transfer into quiescent human and canine polarized intestinal epithelial cells by lentivirus vectors. Journal of Virology, 74(16), 7642-7645.
    • Seppen, J., Barry, S., Lam, G. M., Ramesh, N., & Osborne, W. R. A. (2000). Retroviral preparations derived from PA317 packaging cells contain inhibitors that copurify with viral particles and are devoid of viral vector RNA. Human Gene Therapy, 11(5), 771-775.


    • Wei, M. Q., Lejnieks, D. V., Ramesh, N., Lau, S., Seppen, J., & Osborne, W. R. A. (1999). Sustained gene expression in transplanted skin fibroblasts in rats. Gene Therapy, 6(5), 840-844.


    • Seppen, J., Kimmel, R. J., & Osborne, W. R. A. (1997). Serum-free production, concentration and purification of recombinant retroviruses. BioTechniques, 23(5), 788-790.
    • Seppen, J., Tada, K., Ottenhoff, R., Sengupta, K., Chowdhury, N. R., Chowdhury, J. R., Bosma, P. J., & Oude Elferink, R. P. J. (1997). Transplantation of Gunn rats with autologous fibroblasts expressing bilirubin UDP-glucuronosyltransferase: Correction of genetic deficiency and tumor formation. Human Gene Therapy, 8(1), 27-36.


    • Seppen, J., Steenken, E., Lindhout, D., Bosma, P. J., & Oude Elferink, R. P. J. (1996). A mutation which disrupts the hydrophobic core of the signal peptide of bilirubin UDP-glucuronosyltransferase, an endoplasmic reticulum membrane protein, causes Crigler-Najjar type II. FEBS Letters, 390(3), 294-298.
    • Seppen, J., Tada, K., Hellwig, S., Bakker, C. T. M., Prasad, V. R., Roy Chowdhury, N., Roy Chowdhury, J., Bosma, P. J., & Oude Elferink, R. P. J. (1996). Bilirubin glucuronidation by intact Gunn rat fibroblasts expressing bilirubin UDP-glucuronosyltransferase. Biochemical Journal, 314(2), 477-483.


    • Seppen, J., Jansen, P. L., & Oude Elferink, R. P. J. (1995). Immunoaffinity purification and reconstitution of the human bilirubin/phenol UDP-glucuronosyltransferase family. Protein Expression and Purification, 6(2), 149-154.


    • Bosma, P. J., Seppen, J., Goldhoorn, B., Bakker, C., Oude Elferink, R. P. J., Chowdhury, J. R., Chowdhury, N. R., & Jansen, P. L. M. (1994). Bilirubin UDP-glucuronosyltransferase 1 is the only relevant bilirubin glucuronidating isoform in man. Journal of Biological Chemistry, 269(27), 17960-17964.
    • Seppen, J., Bosma, P. J., Goldhoorn, B. G., Bakker, C. T. M., Chowdhury, J. R., Chowdhury, N. R., Jansen, P. L. M., & Oude Elferink, R. P. J. (1994). Discrimination between Crigler-Najjar type I and II by expression of mutant bilirubin uridine diphosphate-glucuronosyltransferase. Journal of Clinical Investigation, 94(6), 2385-2391.


    • Seppen, J., Ramalho-Santos, J., de Carvalho, A. P., ter Beest, M., Kok, J. W., de Lima, M. C., & Hoekstra, D. (1992). Interaction of clathrin with large unilamellar phospholipid vesicles at neutral pH. Lipid dependence and protein penetration. Biochimica et Biophysica Acta, 1106(1), 209-15.


    • Botschuijver, S., Roeselers, G., Levin, E., Jonkers, D., Welting, O., Heinsbroek, S., de Weerd, H. H., Boekhout, T., Fornai, M., Masclee, A., de Jonge, W., Seppen, J., & van den Wijngaard, R. M. (2017). Fecal Transplantation and Mycobiome Analysis Showing the Relevance of Fungi in Post Stress Visceral Hypersensitivity of Maternal Separated Rats. Gastroenterology, 152(5, Supplement 1), S160. [732]. [details]


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  • Ancillary activities
    No ancillary activities