Dr. ir. J. (Joachim) Goedhart
Faculty of Science
Swammerdam Institute for Life Sciences
Visiting address
- Science Park 904
- Room number: C2.264
Postal address
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Postbus 1212
1000 BE Amsterdam
Contact details
- Profile
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Publications
2024
Goedhart, J. (2024). Studentsourcing-Aggregating and reusing data from a practical cell biology course. PLoS Computational Biology, 20(2), e1011836. https://doi.org/10.1371/journal.pcbi.1011836 [details]- Goedhart, J., & Gadella, T. W. J. (2024). Breaking up the StayGold dimer yields three photostable monomers. Nature Methods, 21(4), 558-559. https://doi.org/10.1038/s41592-024-02223-8 [details]
2023
- Grönloh, M. L. B., Arts, J. J. G., Mahlandt, E. K., Nolte, M. A., Goedhart, J., & van Buul, J. D. (2023). Primary adhered neutrophils increase JNK1-MARCKSL1-mediated filopodia to promote secondary neutrophil transmigration. iScience, 26(8), Article 107406. https://doi.org/10.1016/j.isci.2023.107406 [details]
- Grönloh, M. L. B., Arts, J. J. G., Palacios Martínez, S., van der Veen, A. A., Kempers, L., van Steen, A. C. I., Roelofs, J. J. T. H., Nolte, M. A., Goedhart, J., & van Buul, J. D. (2023). Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM-1. EMBO Reports, 24(1), Article e55483. https://doi.org/10.15252/embr.202255483 [details]
- Kreider-Letterman, G., Castillo, A., Mahlandt, E. K., Goedhart, J., Rabino, A., Goicoechea, S., & Garcia-Mata, R. (2023). ARHGAP17 regulates the spatiotemporal activity of Cdc42 at invadopodia. Journal of Cell Biology, 222(2), Article e202207020. https://doi.org/10.1083/jcb.202207020 [details]
- Mahlandt, E. K., Kreider-Letterman, G., Chertkova, A. O., Garcia-Mata, R., & Goedhart, J. (2023). Cell-based optimization and characterization of genetically encoded location-based biosensors for Cdc42 or Rac activity. Journal of Cell Science, 136(10), Article jcs260802. https://doi.org/10.1242/jcs.260802 [details]
- Mahlandt, E. K., Palacios Martínez, S., Arts, J. J. G., Tol, S., van Buul, J. D., & Goedhart, J. (2023). Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength. eLife, 12, Article RP84364. https://doi.org/10.7554/eLife.84364
- Salas-Lloret, D., Jansen, N. S., Nagamalleswari, E., van der Meulen, C., Gracheva, E., de Ru, A. H., Otte, H. A. M., van Veelen, P. A., Pichler, A., Goedhart, J., Vertegaal, A. C. O., & González-Prieto, R. (2023). SUMO-activated target traps (SATTs) enable the identification of a comprehensive E3-specific SUMO proteome. Science Advances, 9(31), Article eadh2073. https://doi.org/10.1126/sciadv.adh2073 [details]
2022
- Chavez Abiega, S., Grönloh, M. L. B., Gadella, T. W. J., Bruggeman, F. J., & Goedhart, J. (2022). Single-cell imaging of ERK and Akt activation dynamics and heterogeneity induced by G-protein-coupled receptors. Journal of Cell Science, 135(6), Article 259685. https://doi.org/10.1242/jcs.259685 [details]
- Mahlandt, E. K., & Goedhart, J. (2022). Visualizing and Quantifying Data from Time-Lapse Imaging Experiments. In B. Heit (Ed.), Fluorescent Microscopy (pp. 329-348). (Methods in Molecular Biology; Vol. 2440). Humana Press. https://doi.org/10.1007/978-1-0716-2051-9_19 [details]
- Rashid, F-ZM., Mahlandt, E., van der Vaart, M., Boer, D. E. C., Varela Alvarez, M., Henneman, B., Brocken, D. J. W., Voskamp, P., Blok, A. J., Shimizu, T. S., Meijer, A. H., Luijsterburg, M. S., Goedhart, J., Crémazy, F. G. E., & Dame, R. T. (2022). HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein. Nucleic Acids Research, 50(2), Article e10. https://doi.org/10.1093/nar/gkab993 [details]
2021
- Arts, J. J. G., Mahlandt, E. K., Grönloh, M. L. B., Schimmel, L., Noordstra, I., Gordon, E., van Steen, A. C. I., Tol, S., Walzog, B., van Rijssel, J., Nolte, M. A., Postma, M., Khuon, S., Heddleston, J. M., Wait, E., Chew, T. L., Winter, M., Montanez, E., Goedhart, J., & van Buul, J. D. (2021). Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration. eLife, 10, Article e66074. https://doi.org/10.7554/eLife.66074 [details]
- Arts, J. J. G., Mahlandt, E. K., Schimmel, L., Grönloh, M. L. B., van der Niet, S., Klein, B. J. A. M., Fernandez-Borja, M., van Geemen, D., Huveneers, S., van Rijssel, J., Goedhart, J., & van Buul, J. D. (2021). Endothelial Focal Adhesions Are Functional Obstacles for Leukocytes During Basolateral Crawling. Frontiers in Immunology, 12, Article 667213. https://doi.org/10.3389/fimmu.2021.667213 [details]
- Bordes, L., Chavez-Abiega, S., & Goedhart, J. (2021). Imaging of Genetically Encoded FRET-Based Biosensors to Detect GPCR Activity. In G Protein-Coupled Receptor Screening Assays (Vol. 2268, pp. 159-178). (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-1221-7_11
- Botman, D., O'Toole, T. G., Goedhart, J., Bruggeman, F. J., van Heerden, J. H., & Teusink, B. (2021). A yeast FRET biosensor enlightens cAMP signaling. Molecular Biology of the Cell, 32(13), 1229-1240. https://doi.org/10.1091/mbc.E20-05-0319 [details]
- Brandorff, E., Galland, M., & Goedhart, J. (2021). PlotXpress, a webtool for normalization and visualization of reporter expression data [version 1; peer review: 3 approved]. F1000Research, 10, Article 1125. https://doi.org/10.12688/f1000research.73641.1 [details]
- Goedhart, J. (2021). SuperPlotsOfData-a web app for the transparent display and quantitative comparison of continuous data from different conditions. Molecular Biology of the Cell, 32(6), 470-474. https://doi.org/10.1091/mbc.E20-09-0583 [details]
- Goedhart, J., & Rishniw, M. (2021). BA-plotteR - A web tool for generating Bland-Altman plots and constructing limits of agreement. Research in Veterinary Science, 137, 281-286. https://doi.org/10.1016/j.rvsc.2021.05.017 [details]
- Mahlandt, E. K., Arts, J. J. G., van der Meer, W. J., van der Linden, F. H., Tol, S., van Buul, J. D., Gadella, T. W. J., & Goedhart, J. (2021). Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor. Journal of Cell Science, 134(17), Article 258823. https://doi.org/10.1242/jcs.258823 [details]
- Reinhard, N. R., Van Der Niet, S., Chertkova, A., Postma, M., Hordijk, P. L., Gadella, T. W. J., & Goedhart, J. (2021). Identification of guanine nucleotide exchange factors that increase Cdc42 activity in primary human endothelial cells. Small GTPases, 12(3), 226-240. Advance online publication. https://doi.org/10.1080/21541248.2019.1658509 [details]
- van der Linden, F. H., Mahlandt, E. K., Arts, J. J. G., Beumer, J., Puschhof, J., de Man, S. M. A., Chertkova, A. O., Ponsioen, B., Clevers, H., van Buul, J. D., Postma, M., Gadella Jr., T. W. J., & Goedhart, J. (2021). A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. Nature Communications, 12, Article 7159. https://doi.org/10.1038/s41467-021-27249-w [details]
2020
- Chavez-Abiega, S., Mos, I., Centeno, P. P., Elajnaf, T., Schlattl, W., Ward, D. T., Goedhart, J., & Kallay, E. (2020). Sensing Extracellular Calcium - An Insight into the Structure and Function of the Calcium-Sensing Receptor (CaSR). In S. Islam (Ed.), Calcium Signaling (2nd ed., pp. 1031-1063). (Advances in Experimental Medicine and Biology; Vol. 1131). Springer. https://doi.org/10.1007/978-3-030-12457-1_41 [details]
- Goedhart, J. (2020). PlotTwist: A web app for plotting and annotating continuous data. PLoS Biology, 18(1), Article e3000581. https://doi.org/10.1371/journal.pbio.3000581 [details]
- Goedhart, J., & Luijsterburg, M. S. (2020). VolcaNoseR is a web app for creating, exploring, labeling and sharing volcano plots. Scientific Reports, 10(1), Article 20560. https://doi.org/10.1038/s41598-020-76603-3 [details]
- Pietraszewska-Bogiel, A., Joosen, L., Chertkova, A. O., & Goedhart, J. (2020). Not So Dry After All: DRY Mutants of the AT1A Receptor and H1 Receptor Can Induce G-Protein-Dependent Signaling. ACS Omega, 5(6), 2648-2659. https://doi.org/10.1021/acsomega.9b03146 [details]
- van der Weegen, Y., Golan-Berman, H., Mevissen, T. E. T., Apelt, K., González-Prieto, R., Goedhart, J., Heilbrun, E. E., Vertegaal, A. C. O., van den Heuvel, D., Walter, J. C., Adar, S., & Luijsterburg, M. S. (2020). Publisher Correction: The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II. Nature Communications, 11, Article 5734. https://doi.org/10.1038/s41467-020-19643-7
- van der Weegen, Y., Golan-Berman, H., Mevissen, T. E. T., Apelt, K., González-Prieto, R., Goedhart, J., Heilbrun, E. E., Vertegaal, A. C. O., van den Heuvel, D., Walter, J. C., Adar, S., & Luijsterburg, M. S. (2020). The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II. Nature Communications, 11, Article 2104. https://doi.org/10.1038/s41467-020-15903-8 [details]
2019
- Botman, D., de Groot, D. H., Schmidt, P., Goedhart, J., & Teusink, B. (2019). In vivo characterisation of fluorescent proteins in budding yeast. Scientific Reports, 9, Article 2234. https://doi.org/10.1038/s41598-019-38913-z [details]
- Chavez-Abiega, S., Goedhart, J., & Bruggeman, F. J. (2019). Physical biology of GPCR signalling dynamics inferred from fluorescence spectroscopy and imaging. Current opinion in structural biology, 55, 204-211. https://doi.org/10.1016/j.sbi.2019.05.007 [details]
- Goedhart, J., & van Unen, J. (2019). Molecular perturbation strategies to examine spatiotemporal features of Rho GEF and Rho GTPase activity in living cells. Small GTPases, 10(3), 178-186. https://doi.org/10.1080/21541248.2017.1302551 [details]
- Meiresonne, N. Y., Consoli, E., Mertens, L. M. Y., Chertkova, A. O., Goedhart, J., & den Blaauwen, T. (2019). Superfolder mTurquoise2ox optimized for the bacterial periplasm allows high efficiency in vivo FRET of cell division antibiotic targets. Molecular Microbiology, 111(4), 1025-1038. Advance online publication. https://doi.org/10.1111/mmi.14206 [details]
- Postma, M., & Goedhart, J. (2019). PlotsOfData-A web app for visualizing data together with their summaries. PLoS Biology, 17(3), Article e3000202. https://doi.org/10.1371/journal.pbio.3000202 [details]
2018
- Long, Y., Stahl, Y., Weidtkamp-Peters, S., Smet, W., Du, Y., Gadella Jr., T. W. J., Goedhart, J., Scheres, B., & Blilou, I. (2018). Optimizing FRET-FLIM Labeling Conditions to Detect Nuclear Protein Interactions at Native Expression Levels in Living Arabidopsis Roots. Frontiers in Plant Science, 9, Article 639. https://doi.org/10.3389/fpls.2018.00639 [details]
- Mastop, M., Reinhard, N. R., Zuconelli, C. R., Terwey, F., Gadella, T. W. J., van Unen, J., Adjobo-Hermans, M. J. W., & Goedhart, J. (2018). A FRET-based biosensor for measuring Gα13 activation in single cells. PLoS ONE, 13(3), Article e0193705. https://doi.org/10.1371/journal.pone.0193705 [details]
- Mastop, M., Reinhard, N. R., Zuconelli, C. R., Terwey, F., Gadella, T. W. J., van Unen, J., Adjobo-Hermans, M. J. W., & Goedhart, J. (2018). Correction: A FRET-based biosensor for measuring Gα13 activation in single cells. PLoS ONE, 13(4), Article e0195649. https://doi.org/10.1371/journal.pone.0195649
- van Unen, J., Botman, D., Yin, T., Wu, Y. I., Hink, M. A., Gadella, T. W. J., Postma, M., & Goedhart, J. (2018). The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling. BMC Cell Biology, 19, Article 6. https://doi.org/10.1186/s12860-018-0155-2 [details]
2017
- Long, Y., Stahl, Y., Weidtkamp-Peters, S., Postma, M., Zhou, W., Goedhart, J., Sánchez-Pérez, M-I., Gadella, T. W. J., Simon, R., Scheres, B., & Blilou, I. (2017). In vivo FRET–FLIM reveals cell-type-specific protein interactions in Arabidopsis roots. Nature, 548(7665), 97-102. https://doi.org/10.1038/nature23317 [details]
- Mastop, M., Bindels, D. S., Shaner, N. C., Postma, M., Gadella, T. W. J., & Goedhart, J. (2017). Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2. Scientific Reports, 7, Article 11999. https://doi.org/10.1038/s41598-017-12212-x [details]
- Reinhard, N. R., Mastop, M., Yin, T., Wu, Y., Bosma, E. K., Gadella, T. W. J., Goedhart, J., & Hordijk, P. L. (2017). The balance between Gαi-Cdc42/Rac and Gα12/13-RhoA pathways determines endothelial barrier regulation by sphingosine-1-phosphate. Molecular Biology of the Cell, 28(23), 3371-3382. https://doi.org/10.1091/mbc.E17-03-0136 [details]
- Scala, A., Mirabella, R., Goedhart, J., de Vries, M., Haring, M. A., & Schuurink, R. C. (2017). Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness. Plant Molecular Biology, 95(4-5), 399-409. https://doi.org/10.1007/s11103-017-0659-8 [details]
- Vermeer, J. E. M., van Wijk, R., Goedhart, J., Geldner, N., Chory, J., Gadella, T. W. J., & Munnik, T. (2017). In Vivo Imaging of Diacylglycerol at the Cytoplasmic Leaflet of Plant Membranes. Plant and Cell Physiology, 58(7), 1196-1207. https://doi.org/10.1093/pcp/pcx012 [details]
- Zuidscherwoude, M., Dunlock, V-ME., van den Bogaart, G., van Deventer, S. J., van der Schaaf, A., van Oostrum, J., Goedhart, J., In 't Hout, J., Hämmerling, G. J., Tanaka, S., Nadler, A., Schultz, C., Wright, M. D., Adjobo-Hermans, M. J. W., & van Spriel, A. B. (2017). Tetraspanin microdomains control localized protein kinase C signaling in B cells. Science Signaling, 10(478), Article eaag2755. https://doi.org/10.1126/scisignal.aag2755 [details]
2016
- Heemskerk, N., Schimmel, L., Oort, C., van Rijssel, J., Yin, T., Ma, B., van Unen, J., Pitter, B., Huveneers, S., Goedhart, J., Wu, Y., Montanez, E., Woodfin, A., & van Buul, J. D. (2016). F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling. Nature Communications, 7, Article 10493. https://doi.org/10.1038/ncomms10493 [details]
- Raspe, M., Kedziora, K. M., van den Broek, B., Zhao, Q., de Jong, S., Herz, J., Mastop, M., Goedhart, J., Gadella, T. W. J., Young, I. T., & Jalink, K. (2016). siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data. Nature Methods, 13(6), 501-504. https://doi.org/10.1038/NMETH.3836 [details]
- Reinhard, N. R., van Helden, S. F., Anthony, E. C., Yin, T., Wu, Y. I., Goedhart, J., Gadella, T. W. J., & Hordijk, P. L. (2016). Spatiotemporal analysis of RhoA/B/C activation in primary human endothelial cells. Scientific Reports, 6, Article 25502. https://doi.org/10.1038/srep25502 [details]
- van Unen, J., Rashidfarrokhi, A., Hoogendoorn, E., Postma, M., Gadella, T. W. J., & Goedhart, J. (2016). Quantitative Single-Cell Analysis of Signaling Pathways Activated Immediately Downstream of Histamine Receptor Subtypes. Molecular Pharmacology, 90(3), 162-176. https://doi.org/10.1124/mol.116.104505 [details]
- van Unen, J., Stumpf, A. D., Schmid, B., Reinhard, N. R., Hordijk, P. L., Hoffmann, C., Gadella (jr.), T. W. J., & Goedhart, J. (2016). A New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells. PLoS ONE, 11(1), Article e0146789. https://doi.org/10.1371/journal.pone.0146789 [details]
- van Unen, J., Yin, T., Wu, Y. I., Mastop, M., Gadella, T. W. J., & Goedhart, J. (2016). Kinetics of recruitment and allosteric activation of ARHGEF25 isoforms by the heterotrimeric G-protein Gαq. Scientific Reports, 6, Article 36825. https://doi.org/10.1038/srep36825 [details]
2015
- Dattoli, A. A., Hink, M. A., DuBuc, T. Q., Teunisse, B. J., Goedhart, J., Röttinger, E., & Postma, M. (2015). Domain analysis of the Nematostella vectensis SNAIL ortholog reveals unique nucleolar localization that depends on the zinc-finger domains. Scientific Reports, 5, Article 12147. https://doi.org/10.1038/srep12147 [details]
- Klarenbeek, J., Goedhart, J., van Batenburg, A., Groenewald, D., & Jalink, K. (2015). Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated sensors for FLIM, for ratiometry and with high affinity. PLoS ONE, 10(4), Article e0122513. https://doi.org/10.1371/journal.pone.0122513 [details]
- Long, Y., Goedhart, J., Schneijderberg, M., Terpestra, I., Shimotohno, A., Bouchet, B. P., Akhmanova, A., Gadella (jr.), T. W. J., Heidstra, R., Scheres, B., & Blilou, I. (2015). SCARECROW-LIKE23 and SCARECROW jointly specify endodermal cell fate but distinctly control SHORT-ROOT movement. Plant Journal, 84(4), 773-784. https://doi.org/10.1111/tpj.13038 [details]
- Timmerman, I., Heemskerk, N., Kroon, J., Schaefer, A., van Rijssel, J., Hoogenboezem, M., van Unen, J., Goedhart, J., Gadella, T. W. J., Yin, T., Wu, Y., Huveneers, S., & van Buul, J. D. (2015). A local VE-cadherin and Trio-based signaling complex stabilizes endothelial junctions through Rac1. Journal of Cell Science, 128(16), 3041-3054. Advance online publication. https://doi.org/10.1242/jcs.168674 [details]
- van Unen, J., Reinhard, N. R., Yin, T., Wu, Y. I., Postma, M., Gadella, T. W. J., & Goedhart, J. (2015). Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization. Scientific Reports, 5, Article 14693. https://doi.org/10.1038/srep14693 [details]
- van Unen, J., Woolard, J., Rinken, A., Hoffmann, C., Hill, S. J., Goedhart, J., Bruchas, M. R., Bouvier, M., & Adjobo-Hermans, M. J. W. (2015). A Perspective on Studying G-Protein-Coupled Receptor Signaling with Resonance Energy Transfer Biosensors in Living Organisms. Molecular Pharmacology, 88(3), 589-595. Advance online publication. https://doi.org/10.1124/mol.115.098897 [details]
2014
- Bindels, D. S., Goedhart, J., Hink, M. A., van Weeren, L., Joosen, L., & Gadella (jr.), T. W. J. (2014). Optimization of fluorescent proteins. In Y. Engelborghs, & A. J. W. G. Visser (Eds.), Fluorescence Spectroscopy and Microscopy: Methods and Protocols (pp. 371-417). (Methods in Molecular Biology; Vol. 1076). Humana Press. https://doi.org/10.1007/978-1-62703-649-8_16 [details]
- Goedhart, J., Hink, M. A., & Jalink, K. (2014). An introduction to fluorescence imaging techniques geared towards biosensor applications. In J. Zhang, Q. Ni, & R. H. Newman (Eds.), Fluorescent protein-based biosensors: methods and protocols (pp. 17-28). (Methods in Molecular Biology; Vol. 1071). Humana Press. https://doi.org/10.1007/978-1-62703-622-1_2 [details]
- Hamers, D., van Voorst Vader, L., Borst, J. W., & Goedhart, J. (2014). Development of FRET biosensors for mammalian and plant systems. Protoplasma, 251(2), 333-347. Advance online publication. https://doi.org/10.1007/s00709-013-0590-z [details]
- Jezierska, J., Goedhart, J., Kampinga, H. H., Reits, E. A., & Verbeek, D. S. (2014). SCA14 mutation V138E leads to partly unfolded PKCγ associated with an exposed C-terminus, altered kinetics, phosphorylation and enhanced insolubilization. Journal of neurochemistry, 128(5), 741-751. https://doi.org/10.1111/jnc.12491 [details]
- Joosen, L., Hink, M. A., Gadella, T. W. J., & Goedhart, J. (2014). Effect of fixation procedures on the fluorescence lifetimes of Aequorea victoria derived fluorescent proteins. Journal of Microscopy, 256(3), 166-176. https://doi.org/10.1111/jmi.12168 [details]
2013
- Adjobo-Hermans, M. J. W., Crosby, K. C., Putyrski, M., Bhageloe, A., van Weeren, L., Schultz, C., Goedhart, J., & Gadella (jr.), T. W. J. (2013). PLCbeta isoforms differ in their subcellular location and their CT-domain dependent interaction with Galphaq. Cellular Signalling, 25(1), 255-263. Advance online publication. https://doi.org/10.1016/j.cellsig.2012.09.022 [details]
- Gillis, J., Schipper-Krom, S., Juenemann, K., Gruber, A., Coolen, S., van den Nieuwendijk, R., van Veen, H., Overkleeft, H., Goedhart, J., Kampinga, H. H., & Reits, E. A. (2013). The DNAJB6 and DNAJB8 protein chaperones prevent intracellular aggregation of polyglutamine peptides. The Journal of Biological Chemistry, 288(24), 17225-17237. https://doi.org/10.1074/jbc.M112.421685 [details]
- Goedhart, J., van Unen, J., Adjobo-Hermans, M. J. W., & Gadella (jr.), T. W. J. (2013). Signaling efficiency of Gαq through its effectors p63RhoGEF and GEFT depends on their subcellular location. Scientific Reports, 3, Article 2284. https://doi.org/10.1038/srep02284 [details]
2012
- Goedhart, J., von Stetten, D., Noirclerc-Savoye, M., Lelimousin, M., Joosen, L., Hink, M. A., van Weeren, L., Gadella, T. W. J., & Royant, A. (2012). Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nature Communications, 3, 751. https://doi.org/10.1038/ncomms1738 [details]
- von Stetten, D., Noirclerc-Savoye, M., Goedhart, J., Gadella, T. W. J., & Royant, A. (2012). Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K. Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, 68(8), 878-882. https://doi.org/10.1107/S1744309112028667 [details]
2011
- Adjobo-Hermans, M. J. W., Goedhart, J., van Weeren, L., Nijmeijer, S., Manders, E. M. M., Offermanns, S., & Gadella (jr.), T. W. J. (2011). Real-time visualization of heterotrimeric G protein Gq activation in living cells. BMC Biology, 9, Article 32. https://doi.org/10.1186/1741-7007-9-32 [details]
- Goedhart, J., & Gadella (jr.), T. W. J. (2011). Bypassing GPCRs with chemical dimerizers. Chemistry & Biology, 18(9), 1067-1068. https://doi.org/10.1016/j.chembiol.2011.09.004 [details]
- Goedhart, J., van Weeren, L., Adjobo-Hermans, M. J. W., Elzenaar, I., Hink, M. A., & Gadella (jr.), T. W. J. (2011). Quantitative co-expression of proteins at the single cell level - application to a multimeric FRET sensor. PLoS ONE, 6(11), Article e27321. https://doi.org/10.1371/journal.pone.0027321 [details]
- Klarenbeek, J. B., Goedhart, J., Hink, M. A., Gadella, T. W. J., & Jalink, K. (2011). A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range. PLoS ONE, 6(4), Article e19170. https://doi.org/10.1371/journal.pone.0019170 [details]
2010
- Goedhart, J., van Weeren, L., Hink, M. A., Vischer, N. O. E., Jalink, K., & Gadella (jr.), T. W. J. (2010). Bright cyan fluorescent protein variants identified by fluorescence lifetime screening. Nature Methods, 7(2), 137-139. https://doi.org/10.1038/NMETH.1415 [details]
- Luijsterburg, M. S., von Bornstaedt, G., Gourdin, A. M., Politi, A. Z., Moné, M. J., Warmerdam, D. O., Goedhart, J., Vermeulen, W., van Driel, R., & Höfer, T. (2010). Stochastic and reversible assembly of a multiprotein DNA repair complex ensures accurate target site recognition and efficient repair. Journal of Cell Biology, 189(3), 445-463. https://doi.org/10.1083/jcb.200909175 [details]
- Schultz, C., Neef, A. B., Gadella Jr, T. W. J., & Goedhart, J. (2010). Imaging lipids in living cells. Cold Spring Harbor protocols, 2010(7), Article pdb.top83. https://doi.org/10.1101/pdb.top83 [details]
- Schultz, C., Neef, A. B., Gadella Jr, T. W., & Goedhart, J. (2010). Labeling lipids for imaging fixed cells. Cold Spring Harbor protocols, 5(7), Article pdb.prot5458. https://doi.org/10.1101/pdb.prot5458 [details]
- Schultz, C., Neef, A. B., Gadella Jr, T. W., & Goedhart, J. (2010). Labeling lipids for imaging in live cells. Cold Spring Harbor protocols, 5(7), Article pdb.prot5459. https://doi.org/10.1101/pdb.prot5459 [details]
2009
- Kremers, G. J., & Goedhart, J. (2009). Visible fluorescent proteins for FRET. In T. W. J. Gadella (Ed.), FRET and FLIM techniques (pp. 171-223). (Laboratory techniques in biochemistry and molecular biology; No. 33). Elsevier. https://doi.org/10.1016/S0075-7535(08)00005-3 [details]
- Shcherbo, D., Souslova, E. A., Goedhart, J., Chepurnykh, T. V., Gaintzeva, A., Shemiakina, I. I., Gadella, T. W. J., Lukyanov, S., & Chudakov, D. M. (2009). Practical and reliable FRET/FLIM pair of fluorescent proteins. BMC Biotechnology, 9, 24. https://doi.org/10.1186/1472-6750-9-24 [details]
- Vermeer, J. E. M., Thole, J. M., Goedhart, J., Nielsen, E., Munnik, T., & Gadella, T. W. J. (2009). Imaging phosphatidylinositol 4-phosphate dynamics in living plant cells. Plant Journal, 57(2), 356-372. https://doi.org/10.1111/j.1365-313X.2008.03679.x [details]
2008
- Adjobo-Hermans, M. J. W., Goedhart, J., & Gadella, T. W. J. (2008). Regulation of PLCβ1a membrane anchoring by its substrate phosphatidylinositol (4,5)-bisphosphate. Journal of Cell Science, 121(22), 3770-3777. https://doi.org/10.1242/jcs.029785 [details]
- Kremers, G. J., van Munster, E. B., Goedhart, J., & Gadella, T. W. J. (2008). Quantitative lifetime unmixing of multiexponentially decaying fluorophores using single-frequency fluorescence lifetime imaging microscopy. Biophysical Journal, 95(1), 378-389. https://doi.org/10.1529/biophysj.107.125229 [details]
- Schenning, M., Goedhart, J., Gadella (jr.), T. W. J., Avram, D., Wirtz, K. W. A., & Snoek, G. T. (2008). The anti-apoptotic activity associated with phosphatidylinositol transfer protein α activates the MAPK and Akt/PKB pathway. Biochimica et Biophysica Acta-Molecular Cell Research, 1783(10), 1700-1706. https://doi.org/10.1016/j.bbamcr.2008.04.014 [details]
2021
- Chavez-Abiega, S., Grönloh, M. L. B., Gadella, T. W. J., Bruggeman, F. J., & Goedhart, J. (2021). Heterogeneity and dynamics of ERK and Akt activation by G protein-coupled receptors depend on the activated heterotrimeric G proteins. (v3 ed.) BioRxiv. https://doi.org/10.1101/2021.07.27.453948 [details]
2018
- Goedhart, J. (2018). Statistics: prevent P-value parroting. Nature, 554(7690), 31. https://doi.org/10.1038/d41586-018-01314-9 [details]
2015
- Timmerman, I., Heemskerk, N., Kroon, J., Schaefer, A., van Rijssel, J., Hoogenboezem, M., van Unen, J., Goedhart, J., Gadella, T. W. J., Yin, T., Wu, Y., Huveneers, S., & van Buul, J. D. (2015). A local VE-cadherin and Trio-based signaling complex stabilizes endothelial junctions through Rac1: Correction. Journal of Cell Science, 128(18), 3514. https://doi.org/10.1242/jcs.179424
2010
- Schultz, C., Neef, A. B., Gadella Jr, T. W. J., & Goedhart, J. (2010). Transfection of cells with DNA encoding a visible fluorescent protein-tagged lipid-binding domain. Cold Spring Harbor protocols, 2010(7), Article pdb.top83. https://doi.org/10.1101/pdb.prot5457 [details]
2009
- Goedhart, J., & Gadella (jr.), T. W. J. (2009). Fluorescence resonance energy transfer imaging of PKC signalling in living cells using genetically encoded fluorescent probes. Journal of the Royal Society Interface, 6(S1), S27-S34. https://doi.org/10.1098/rsif.2008.0340.focus [details]
2008
- Verbeek, D. S., Goedhart, J., Bruinsma, L., Sinke, R. J., & Reits, E. A. (2008). PKCγ mutations in spinocerebellar ataxia type 14 affect C1 domain accessibility and kinase activity leading to aberrant MAPK signaling. Journal of Cell Science, 121(14), 2339-2349. https://doi.org/10.1242/jcs.027698 [details]
2025
2024
2023
2022
2020
- Mastop, M. (2020). Developing, optimizing and using FRET-based biosensors to elucidate G protein signaling. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Van Geel, O. (2020). When cells respond to light: All you need is LOV. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
2019
2021
- Mahlandt, E. & Goedhart, J. (3-9-2021). TIRF imaging data of neutrophils migrating underneath endothelial cells. Zenodo. https://doi.org/10.5281/zenodo.5410324
- Mouton, G., Grönloh, M., van Buul, J. & Goedhart, J. (2021). An endothelial monolayer with leukocytes annotated for classification with bounding boxes for YOLO. Zenodo. https://doi.org/10.5281/zenodo.5024988
- Rashid, F., Mahlandt, E., van der Vaart, M., Boer, D., Solari, J., Shimizu, T., Henneman, B., Varela, M., Brocken, D., Voskamp, P., Luijsterburg, M. S., Blok, A. J., Meijer, A. H., Goedhart, J., Cremazy, F. & Dame, R. (2021). Data supporting the paper "HI-NESS: A family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein". 4TU.ResearchData. https://doi.org/10.4121/14892519.v1
2020
- van der Weegen, Y., González-Prieto, R., Goedhart, J., Vertegaal, A. C. O. & Luijsterburg, M. S. (2020). Mass spectrometry data for interactive volcano plots - van der Weegen et al., 2020. Zenodo. https://doi.org/10.5281/zenodo.3712919
- van der Weegen, Y., González-Prieto, R., Goedhart, J., Vertegaal, A. C. O. & Luijsterburg, M. S. (2020). Mass spectrometry data for interactive volcano plots - by van der Weegen et al.. Zenodo. https://doi.org/10.5281/zenodo.3713174
2018
- Botman, D., Groot, D., Schmidt, P., Goedhart, J. & Teusink, B. (22-10-2018). In vivo characterisation of fluorescent proteins in budding yeast.. Zenodo. https://doi.org/10.5281/zenodo.1468183
- Goedhart, J. (29-4-2018). Material related to the blog that reports on the parrot LUT. Zenodo. https://doi.org/10.5281/zenodo.1211690
2017
- Goedhart, J. (6-10-2017). Test data in spreadsheet format. Zenodo. https://doi.org/10.5281/zenodo.1003223
- Mastop, M., Bindels, D., Gadella, D. & Goedhart, J. (16-5-2017). Absorption and Emission spectra of fluorescent proteins. Zenodo. https://doi.org/10.5281/zenodo.580169
- van Unen, J., Botman, D., Hink, M., Postma, M. & Goedhart, J. (2017). The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling. Zenodo. https://doi.org/10.5281/zenodo.1092754
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Goedhart, J. (2024). Studentsourcing-Aggregating and reusing data from a practical cell biology course. PLoS Computational Biology, 20(2), e1011836.